摘要
[目的]优化杠板归多糖提取工艺条件,为杠板归药材的进一步开发研究提供参考。[方法]采用苯酚-硫酸法测定杠板归中多糖的含量;通过单因素试验,根据响应面法设计试验,选取其最佳提取工艺。[结果]最佳提取工艺为料液比1∶60、提取时间1.84 h、提取次数3次,此提取条件下多糖得率最高。[结论]该工艺稳定、可行、提取率高,可为其进一步开发利用提供理论依据。
[Objective] The research aimed to optimize the extraction process of Polygonum perfoliatum polysaccharide,which provides a reference for the further development of the medicinal material.[Methods]The content of P. perfoliatum polysaccharide was determined by phenolsulfuric acid method. Through the single factor test,the test was designed according to the response surface method,and the best extraction process was selected.[Results]Optimum extraction conditions for the polysaccharide were： the solid-liquid ratio was 1： 60,the extracting time were 1.84 hours,and the extraction times were 3 times.The yield of polysaccharide was the highest under this extraction condition. [Conclusion]The process was stable,feasible and high extraction rate,which can provide a reference and further development for the research of P.perfoliatum polysaccharide.
作者
杨菁
蒋乐巧
谢娟
聂晓薇
朱丹
YANG Jing;JIANG Le-qiao;XIE Juan(School of Pharmacy,Guiyang College of Traditional Chinese Medicine,Guiyang,Guizhou 550002)
出处
《安徽农业科学》
CAS
2018年第23期136-138,共3页
Journal of Anhui Agricultural Sciences
基金
贵阳中医学院大学生创新创业训练计划项目(贵中医大创合字[2015]12号)
关键词
杠板归
多糖
响应面法
提取工艺
优化
Polygonum perfoliatum L.
Polysaeeharide
Response surface method
Extraction process
Optimization
