摘要
为研究铜绿假单胞菌YY24硝化、反硝化功能基因及催化过程,分别对关键酶基因(amoA、napA、narG、nirK、nirS、cnorB、qnorB、nosZ、nifH)进行PCR扩增、鉴定及测序,通过琼脂糖凝胶电泳确定目的基因片段。研究结果表明,amoA基因为铜绿假单胞菌YY24硝化关键酶氨单加氧酶的编码基因;铜绿假单胞菌YY24存在膜结合硝酸盐还原酶;亚硝酸还原酶的类型为细胞色素cd1型亚硝酸盐还原酶nirS;铜绿假单胞菌YY24存在一氧化氮还原酶norB,能够将NO催化转化为N2O,其基因类型为qnorB;铜绿假单胞菌YY24中存在nosZ基因。
The functional genes including amoA,napA,narG,nirK,nirS,cnorB,qnorB,nosZ,and nifH were amplified,and sequenced by restriction enzyme digestion and PCR and the target gene fragment was analyzed by agarose gel electrophoresis in order to evaluate the genes of denitrification and nitrification function and to understand denitrification process of Pseudomonas aeruginosa YY24.The results showed that amoA gene is the coding gene of ammonia monooxygenase in YY24.There was membrane-bound nitrate reductase as cytochrome cd1 reductase nirS and nosZ gene in YY24.It was found that nitric oxide reductase catalyzed NO to N2 O and had gene qnorB in YY24.
作者
刘兴
李连星
薄香兰
陈继楚
周胜杰
王军
王立红
陈成勋
LIU Xing;LI Lianxing;BO Xianglan;CHEN Jichu;ZHOU Shengjie;WANG Jun;WANG Lihong;CHEN Chengxun(Tianjin Key Laboratory of AqumEcology and Aquaculture,Department of Fishery Science,Agricultural University,Tianjin 300384,China;Tianjin Lida Water Resources Development Co.,Ltd,Tianjin 300280,China;The Key Laboratory of Microbial Preparation Enterprise in Tianjin,Dingzheng Animal Pharmaceutical Co.,Ltd,Tianjin 300383,China)
出处
《水产科学》
CAS
CSCD
北大核心
2018年第4期475-483,共9页
Fisheries Science
基金
国家自然科学基金(面上)资助项目(31270456)
天津市水产产业技术体系创新团队项目(ITTFRS2017001)
天津市科技支撑计划重大项目(12ZCDZNC05900)
天津市应用基础与前沿技术研究计划(重点项目)(13JCZDJC29200)
天津市教委一般项目(20140626)
关键词
硝化
反硝化
功能基因
铜绿假单胞菌
nitrification
denitrification
functional gene
Pseudomonasaeruginosa
