摘要
以大和锦叶片为外植体材料,研究不同消毒措施、不同激素浓度配比对大和锦愈伤组织诱导、分化新芽的影响,以期为大和锦快速再生体系的建立,改良大和锦提供一定的参考依据。结果表明,最佳的消毒方式为健康的大和锦叶片用无菌水冲洗1h,75%乙醇浸泡30s,1%次氯酸钠浸泡17min,污染率低至10.00%;诱导大和锦叶片愈伤组织的最佳培养基为MS+3.0mg·L^(-1)6-BA+0.1mg·L^(-1) NAA+1.0mg·L^(-1) KT;大和锦愈伤组织分化新芽的最佳培养基为MS+3.0mg·L^(-1)6-BA+0.2mg·L^(-1) NAA。
With Echeveria purpusorum Berger leaves as explant materials,this study investigated the effect of different disinfection measures and different hormone concentration ratios on callus induction and bud differentiation of Echeveria purpusorum Berger,so as to provide a certain reference for the establishment of a rapid regeneration system of Echeveria purpusorum Berger and the modification of Echeveria purpusorum Berger.The results showed that the optimal disinfection measure was washing with sterile water for 1 h,immersion with75% ethanol for 30 sand immersion with 1% sodium hypochlorite for 17 min for healthy Echeveria purpusorum Berger leaves,with a contamination rate of 10.00%.The optimal culture medium for inducing callus of Echeveria purpusorum Berger was MS＋3.0 mg·L^-16-BA＋0.1 mg·L^-1 NAA＋1.0 mg·L^-1 KT,and the optimal culture medium for differentiating callus to buds was MS＋3.0 mg·L^-16-BA＋0.2 mg·L^-1 NAA.
作者
石振杰
金一锋
陈阳
汪迅
程美玲
孙华山
SHI Zhen-jie;JIN Yi-feng;CHEN Yang;WANG Xun;CHENG Mei-ling;SUN Hua-shan(College of Life Science and Agriculture,Heilongjiang Provincial Key Laboratory of Resistance Gene Engineering and Preservation of Biodiversity in Cold Areas,Qiqihar University,Qiqihar 161006,China;College of Horticulture and Garden,Northeast Agricultural University,Harbin 150030,China)
出处
《黑龙江农业科学》
2018年第7期39-42,共4页
Heilongjiang Agricultural Sciences
基金
国家自然科学基金资助项目(31701958)
黑龙江省大学生创新创业资助项目(201610221018)
黑龙江省高等教育学会"十三五"高等教育科研课题资助项目(16Q154
16Q159)
关键词
大和锦
愈伤组织
消毒措施
Echeveria purpusorum Berger；callus；disinfection measures
