摘要
试验旨在研究梅山猪白细胞分化抗原14(cluster of differentiation antigen 14,CD14)基因多态性及其对部分免疫指标和繁殖性能的遗传效应。采用PCR-RFLP方法对梅山猪CD14基因-61bp处多态性进行分析,同时对部分重要细胞因子(IL-1β、IL-4、IL-6、IL-8、IL-10、IFN-γ、TNF-α和TGF-β1)水平及繁殖性能(总产仔数、断奶仔猪数、初生窝重和断奶窝重)进行测定,并分析CD14基因多态性与部分免疫指标和繁殖性能的相关性。结果显示,梅山猪CD14基因-61bp处经BamHⅠ酶切后共检测到3种基因型,分别定义为:AA、AG和GG;χ2适合性检验显示,梅山猪处于Hardy-Weinberg平衡状态(P>0.05);多态信息含量(PIC)分析显示,梅山猪群体处于中度多态。关联分析结果表明,AA和AG基因型个体的IL-10水平显著高于GG基因型个体(P<0.05);在初产母猪中,3种基因型间的繁殖性能均无显著差异(P>0.05),而在经产母猪中,AA和AG基因型个体的断奶窝重显著高于GG基因型个体(P<0.05)。本试验结果表明,基于CD14基因-61bp处多态性对梅山猪进行分子选育时,无论是一般抗病力还是繁殖性能,GG基因型可能均为不利基因型,可以将CD14基因-61bp处作为一个潜在的遗传标记进行深入系统的验证和分析。
This study was aimed to investigate the genetic effect of cluster of differentiation antigen 14（CD14）gene polymorphism on partial immune indexes and reproductive performance in Meishan pigs.The genetic polymorphism of CD14 gene-61 bp locus in Meishan pigs was detected by PCR-RFLP method,the level of the partial vital cytokines（IL-1β,IL-4,IL-6,IL-8,IL-10,IFN-γ,TNF-αand TGF-β1）and reproductive performances（total litter size,number of weaning piglets,birth litter weight and weaning litter weight）were determined,and the association of CD14 gene polymorphism with these immune indexes and reproductive performance was analyzed.The results showed that the-61 bp locus of CD14 gene in Meishan pigs was digested by BamH Ⅰ,and three genotypes were detected named as AA,AG and GG,respectively.The Chisquare test results showed that the genotype distribution of Meishan pigs were in Hardy-Wein-berg equilibrium（P0.05）,the polymorphism information content（PIC）were moderate polymorphism in Meishan pigs.The correlation analysis indicated that the level of IL-10 of AG and GG genotypes individuals was significantly higher than that of GG genotype individuals（P0.05）.There was no significant difference of reproductive performances among three genotypes in primiparous sow（P0.05）,while the weaning litter weight of AA and AG genotypes individuals was significantly higher than that of GG genotype individuals in multiparous sows（P0.05）.The results of this experiment showed that GG genotype might be unfavorable genotype both in general disease resistance and reproductive performance when Meishan pigs were molecularly selected based on the-61 bp locus of CD14 gene,and the-61 bp locus of CD14 gene could be used as a potential genetic marker for further systematic verification and analysis.
作者
杜雨露
魏宗友
叶兰
包文斌
吴圣龙
DU Yulu;WEI Zongyou;YE Lan;BAO Wenbin;WU Shenglong(College of Animal Science and Technology,Yangzhou University,Yangzhou 225009,China;Taicang Animal Husbandry and Veterinary Station,Taicang 215413,China)
出处
《中国畜牧兽医》
CAS
北大核心
2018年第7期1898-1904,共7页
China Animal Husbandry & Veterinary Medicine
基金
国家科技支撑计划(2015BAD03B01)
苏州市科技局攻关项目(SNG201628)
江苏省农业三新工程(SXGC[2017]302)
扬州大学中青年学术带头人
关键词
猪
CD14基因
免疫指标
繁殖性能
pig
CD14 gene
immune indexes
reproductive performance
