摘要
目的评价低频超声联合两性霉素B纳米粒对白念珠菌生物膜的体外协同杀菌效果评价。方法采用双乳化法制备两性霉素B纳米粒(amphotericin B-loaded nanoparticles,AmB-NPs)。XTT减低法评价经超声与AmB-NPs联合作用后对成熟生物膜活性的影响,激光共聚焦显微镜观察生物膜形态学改变,并检测生物膜分泌蛋白酶和磷脂酶活力。结果与对照组及游离AmB药物相比,超声联合AmB-NPs能明显降低生物膜活性(P<0.01);生物膜厚度变薄,结构疏松,蛋白酶和磷脂酶活力明显下降。结论低频超声联合AmB-NPs对白念珠菌生物膜有显著的协同抗菌作用。
Objective To evaluate the synergistic fungicidal effect of low frequency ultrasound combined with amphotericin B-loaded nanoparticles（AmB-NPs）on C.albicans biofilmin vitro.Method AmB-NPs were prepared by apolylactic-coglycolic acid（PLGA）double emulsion method.XTT reduction method was performed to evaluate the effect of ultrasound with AmB-NPs on the activity of mature biofilm.The morphology of biofilm was observed by laser scanning confocal microscope.The protease and phospholipase activities of biofilm were detected by UV spectrophotometer.Results The particle size and shape of AmB-NPs were uniform.The biofilm activity of C.albicans decreased significantly after the joint action of ultrasonic irradiation and AmB-NPs compared with the control group（P〈0.01）.Simultaneously,the biofilm became thin and loose,with significant decreased activities of protease and phospholipase aenzyme.Conclusion Low-frequency ultrasound combined with AmB-NPs showed significant synergistic antifungal effect against C.albicans biofilmin vitro.
作者
杨敏
谢霜
董宇
杜永洪
李岱容
YANG Min 1 , XIE Shuang 1 , DONG Yu 1 , DU Yong-hong 1 , LI Dai-rong 2(1.State Key Laboratory of Ultrasound Engineering in Medicine Co-Founded by Chongqing and the Ministry of Science and Technology, College of Biomedical Engineering, Chongqing Key Laboratory of Biomedical Engineering, Chongqing Medical University, Chongqing Collaborative Innovation Center for Minimally-invasive and Noninvasive Medicine, Chongqing 400016, China;2.First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China)
出处
《中国真菌学杂志》
CSCD
2018年第3期134-138,共5页
Chinese Journal of Mycology
基金
重庆市基础科学与前沿技术研究专项一般项目(csct2016jcyjA0098)
重庆市社会事业与民生保障专项一般项目(cstc2016shmszx130029)
关键词
低频超声
白念珠菌生物膜
两性霉素B
纳米粒
协同抗菌
low frequency ultrasound
C. albicans biofilm
amphotericin B
nanoparticles
synergistic fungicidal
