摘要
对羟基苯甲酸(4-HBA)是导致葡萄连作障碍产生的重要自毒物质。本试验以‘贝达’葡萄实生苗为试材,选取发芽后长势一致的实生苗进行4-HBA胁迫处理,研究自毒物质对葡萄植株生长及根系分泌特性的影响。结果表明,250μmol/L、500μmol/L和1000μmol/L的4-HBA处理对‘贝达’葡萄的水培幼苗生长表现出不同程度的抑制作用,且随着处理浓度增加抑制作用增强。葡萄根系SOD和PAL酶活性随着处理时间的延长呈现出先上升后下降的趋势,且均在48 h达到峰值;MDA含量增加,96 h高浓度处理根系MDA含量最高。外源4-HBA处理导致根系分泌的4-HBA、肉桂酸含量减少,水杨酸、阿魏酸却显著增加,总酚酸含量在4-HBA为250μmol/L时降低,500μmol/L和1000μmol/L时增高,且与对照达到显著差异。
4-hydroxybenzoic acid（4-HBA） is an important autotoxin which led to grapevine replant disease. Used ＇Beta＇（Vitis riparia ×Vitis labrusca） seedlings as materials in the experiment, the same growth seedling after germination was selected and treated by 4-HBA. The experiment aim was to study the effect of exogenous 4-HBA on grapevine growth and root exudating characteristic. The results showed that seedlings were suppressed at varying degrees under 4-HBA treatment of 250 μmol/L, 500 μmol/L and 1000 μmol/L concentrations. With the increase of treatment concentration, the inhibition effect was enhanced. SOD and PAL enzymatic activity first increased and then decreased with the processing time increase, and maximum appeared at 48 h after treatment. MDA content increased with the enhance of treatment concentration, and the maximum appeared at 96 h under high 4-HBA concentration. The contents of 4-HBA and cinnamic acid decreased, while salicylic acid and ferulic acid content increased after 4-HBA treatment. The total phenolic content decreased when 4-HBA concentration 250 μmol/L, and increased at 500 μmol/L and 1000 μmol/L. There were significant differences between treatments and the control.
作者
孙鑫
刘倩文
李坤
郭修武
郭印山
刘镇东
SUN Xin,LIU Qianwen,LI kun,GUO Xiuwu,GUO Yinshan,LIU Zhendong(College of Horticulture, Shenyang Agricultural University,Shenyang 110161,China)
出处
《中外葡萄与葡萄酒》
2018年第4期12-17,共6页
Sino-Overseas Grapevine & Wine
基金
国家自然科学基金项目(31101503
31572076)
国家现代农业产业技术体系建设专项资助项目(CARS-29-yc-6)
辽宁省教育厅一般项目(2015493)
