摘要
目的观察保元排毒丸对单侧输尿管梗阻(UUO)大鼠TGF-β1、Smad3、Smad7表达的影响。方法 48只大鼠随机分为假手术组,UUO组,厄贝沙坦组,保元排毒丸低、中、高剂量组,每组8只。假手术组和UUO组大鼠灌胃生理盐水,其他组大鼠灌胃相应药物14 d。末次灌胃24 h后,Masson染色观察肾组织病理变化,Western blot、RTPCR分别检测TGF-β1、Smad3、Smad7蛋白、mRNA表达。结果与UUO组比较,保元排毒丸各剂量组和厄贝沙坦组肾纤维化程度明显减轻(P<0.01),TGF-β1、Smad3表达明显降低(P<0.05),Smad7表达明显升高(P<0.05)。结论保元排毒丸可通过下调TGF-β1、Smad3表达,上调Smad7表达来延缓UUO大鼠肾纤维化。
AIM To investigate the effects of Baoyuan Paidu Pills（ BPP） on the expressions of TGF-β1,Smad3 and Smad7 in rats with unilateral ureteral obstruction（ UUO）. METHODS Forty-eight rats were randomly divided into six groups,sham-operation group,UUO group,irbesatan group,high-dose BPP group,mediumdose BPP group,low-dose BPP group,with eight rats in each group. The rats in the sham-operation group and UUO group were intragastrically administrated with normal saline,which in the other groups were intragastrically administrated with corresponding drugs for fourteen days. The pathological changes of renal tissue were observed by Masson staining after 24 h of last intragastric administration. The protein and mRNA expressions of TGF-β1,Smad3 and Smad7 were detected by Western blot and RT-PCR,respectively. RESULTS The various BPP dose groups and irbesatan group displayed their superiority to the UUO group in terms of significantly reduced renal fibrosis degree（ P 〈 0. 01）,significantly decreased expressions of TGF-β1 and Smad3（ P 〈 0. 05）,and significantly increased expression of Smad7（ P 〈 0. 01）. CONCLUSION BPP can postpone renal fibrosis in UUO rats by down-regulating the expressions of TGF-β1,Smad3,and up-regulating the expression of Smad7.
作者
王身菊
张玲
朱美凤
唐丽君
郑宏香
陈岱
WANG Shen-ju;ZHANG Ling;ZHU Mei-feng;TANG Li-jun;ZHENG Hong-xiang;CHEN Dai(Changzhou TCM Hospital Affiliated to Nanjing University of Chinese Medicine,Changzhoo 213000,China)
出处
《中成药》
CAS
CSCD
北大核心
2018年第7期1463-1467,共5页
Chinese Traditional Patent Medicine
基金
江苏省常州市科技局项目(CJ20160042)
