摘要
目的分析常见的非人源细胞系种间交叉污染情况。方法收集160例常见的非人源细胞系,提取基因组DNA,通过PCR法鉴定其来源种属,判断是否存在种属间的交叉污染。对可疑细胞进一步通过染色体分析确认其来源种属。结果 160例非人源细胞中,有6个细胞系的种属鉴定结果与预期种属不符,其中1例大鼠来源细胞混入了人来源细胞,其余5例种属不正确,认定为错误细胞。结论细胞系的种属鉴定是细胞质量控制的不可或缺的环节,只有经过完整质控的细胞才能作为实验材料,用于科学研究和应用。
Objective To analyze interspecies cross-contamination of 160 non-human cell lines. Methods One hundred and sixty common non-human cell lines were collected and their species were identified by PCR. For the suspicious cells,chromosome analysis was further used to confirm their species. Results Six in 160 non-human cell lines were cross-contaminated. A rat cell line was mixed by a human cell line,and 5 were totally cross-contaminated,and were indentified as wrong species. Conclusions Species identification is an indispensable part of cell quality control. Each cell line should undergo a full QA（ Quality Assurance） assessment before it is used for research.
作者
卞晓翠
杨振丽
左春霞
周芳颖
刘玉琴
BIAN Xiao-cui;YANG Zhen-li;ZUO Chun-xia;ZHOU Fang-ying;LIU Yu-qin(L Dept.of Patholog;Cell Resource Center,Institute of Basic Medical Sciences CAMS,School of Basic Medicine PUMC,Beijing 100005,China)
出处
《基础医学与临床》
CSCD
2018年第5期632-637,共6页
Basic and Clinical Medicine
基金
国家科技基础条件平台(NSTI-CR17)
关键词
非人源细胞系
细胞培养
质量控制
种属鉴定
non-human cell lines
cell culture
quality control
species identification
