摘要
猪流行性腹泻(porcine epidemic diarrhea,PED)和猪传染性胃肠炎(transmissible gastroenteritis,TGE)是引起仔猪严重腹泻的两种高度接触性传染病。本文将实验室已有的两株针对TGEV和PEDV的单链抗体(single chain fragment variable,scFv)进行大量表达和纯化,将纯化后的scFv应用到发生疫病的猪场。同时比较了scFv、scFv与奶粉联合使用对仔猪病毒性腹泻的治疗效果,结果证实scFv单独使用后仔猪存活率达到65.0%,scFv与奶粉联合使用后仔猪存活率达到72.7%。本研究探索了一种利用scFv治疗PEDV和TGEV感染的新的给药方法。
Porcine epidemic diarrhea(PED) and transmissible gastroenteritis(TGE) are highly contagious and infectious diseases of piglets. In this study, we test the therapy effect of scFvs targeting PEDV and TGEV. The scFvs were expressed, purified and applied to a pig farm. The therapy effect of scFvs group and scFvs-milk group were analyzed. The results indicated that scFv group and scFvs-milk group increased the viability of piglets to 65.0% and 72.7%, respectively.Our research provided a novel way to treat viruses induced diarrhea on piglets.
作者
张凡庆
陈玉雪
杨亮
龚建培
朱建国
ZHANG Fanqing;CHEN Yuxue;YANG Liang;GONG Jianpei;ZHU Jianguo(Shanghai Key Laboratory of Veterinary Biotechnology,College of Agriculture and Biology,Shanghai Jiaotong University,Shanghai 200240,China;Shanghai Frontan Animal Health Corp.,Shanghai 210502,China;Zhejiang Hisun Pharmaceutical Corp.,Taizhou 318099,China)
出处
《中国猪业》
2018年第6期43-45,48,共4页
China Swine Industry
基金
防治仔猪腹泻的猪源性单链抗体口服制剂研制及应用[沪农科攻字(2015)第1-8号]
上海市生猪现代农业产业技术体系资助[沪农科产字(2018)第6号]