摘要
小麦高分子量麦谷蛋白亚基(HMW-GS)组成与面包品质密切相关。为了从目前经常使用的一些HMW-GS检测方法中选择满足试验要求的最佳方法,笔者总结了HMW-GS组成,以及利用十二烷基硫酸钠聚丙烯酰胺凝胶电泳(SDS-PAGE),反相高效液相色谱(RP-HPLC)和聚合酶链式反应(PCR)3种方法检测小麦HMW-GS组成的研究进展,讨论了3种方法检测小麦HMW-GS组成的优缺点,指出SDSPAGE和PCR方法适合常规育种材料或栽培小麦品种中HMW-GS的检测,双向电泳或RP-HPLC方法适合检测含有远缘遗传物质的小麦或近缘种属中HMW-GS的检测。最后展望了SDS-PAGE和PCR方法在小麦分子标记辅助育种中应用前景。
The composition of high-molecular-weight glutenin subunit（HMW-GS） of wheat is closely relatedto the quality of bread. To select the most suitable detection method of HMW-GS to meet the test requirements,the authors introduced the composition of HMW-GS in wheat and the progress on detecting the composition ofHMW-GS with three methods, including sodium dodecyl sulfate polyacrylamide gel electrophoresis（SDS-PAGE）, reverse phase high-performance liquid（RP-HPLC） and polymerase chain reaction（PCR）. The authorsdiscussed the advantages and disadvantages of the three methods, pointed out that SDS-PAGE and PCRmethods were suitable for the HMW-GS detection in conventional breeding materials and cultivated wheat,and two-dimensional gel electrophoresis and RP-HPLC were suitable for the HMW-GS detection in the wheatwith distant genetic material or the related species. At last, we proposed the application prospect of SDS-PAGE and PCR in the molecular marker assisted breeding of wheat.
作者
高振贤
李亚青
田国英
单子龙
张朋伟
李辉利
常旭虹
Gao Zhenxian;Li Yaqing;Tian Guoying;Shan Zilong;Zhang Pengwei;Li Huili;Chang Xuhong(Shijiazhuang Academy of Agricultural and Forestry Sciences,Shijiazhuang 050041;Institute of Crop Sciences,Chinese Academy of Sciences,Beijing 100081)
出处
《中国农学通报》
2018年第16期35-41,共7页
Chinese Agricultural Science Bulletin
基金
国家重点研发计划"小麦优质高产品种筛选及其配套栽培技术"(2016YFD0300407)
关键词
小麦
高分子量麦谷蛋白
检测方法
分子标记
育种
wheat
high-molecular-weight glutenin subunit
detection method
molecular marker
breeding
