摘要
目的探讨梓醇对顺铂诱导大鼠卵巢颗粒细胞损伤的保护作用及作用机制。方法收集大鼠卵巢颗粒细胞,经原代培养后设为对照组、模型组(顺铂50 nmol/L)和梓醇50、100、200μmol/L组,各组细胞按相应方式进行处理。采用MTT法测定细胞活力;流式细胞术Annexin V/PI双染法考察细胞凋亡情况;免疫组化法观察细胞增殖能力,WB法定量细胞凋亡蛋白的分子水平。结果顺铂可显著抑制卵巢颗粒细胞活力、增殖,诱导细胞凋亡;在给予梓醇进行干预后,大鼠卵巢颗粒细胞活力增强、增殖上调,细胞凋亡减弱;经顺铂进行刺激损伤,卵巢颗粒细胞中caspase-3、caspase-9的活化蛋白表达量显著上升,而不同浓度的梓醇可显著下调细胞中cleaved caspase-3、cleaved caspase-9的蛋白表达量。结论梓醇可以改善大鼠细胞活力、提高细胞增殖和抗凋亡能力,对顺铂诱导的卵巢颗粒细胞损伤产生明显的保护作用。
Objective： To investigate protective effects of catalpolon on apoptosis of rat ovarian granulosa cells induced by cisplatin, and explore its mechanisms. Methods Rat ovarian granulosa cells were cultured and divided into control group, model group（cisplatin 50 nmol/L）, and catalpol（50, 100, and 200 μmol/L） groups. Then cells in each group were treated in the corresponding ways. Cell viability was determined by MTT method. Apoptosis was detected by double staining flow cytometry Annexin V/PI. Western blotting method was used to detect expressions of apoptosis related protein. Results Cisplatin could significantly decrease the viability of rat ovarian granulosa cells and induce apoptosis. After treated by catalpol, the activity of ovarian granulosa cells was increased, proliferation was up-regulated, and apoptosis was decreased. After treated by cisplatin, the expressions of the active form of cleaved caspase-3 and cleaved caspase-9 protein were significantly increased. Catalpol could significantly decrease the expressions of cleaved caspase-3 and cleaved caspase-9 in cells. Conclusion The addition of catalpol to rat ovarian granulosa cells can significantly change the anti-apoptotic and anti-oxidative ability, and play a protective role in the apoptosis induced by cisplatin. Catalpol can improve cell viability, enhance cell proliferation and anti apoptosis ability, and protect apoptosis of ovarian granulosa cells in rats induced by cisplatin.
作者
张汝仙
王花敏
程学敏
赵莎
张晶
ZHANG Ru-xian;WANG Hua-min;CHENG Xue-min;ZHAO Sha;ZHANG Jing(Xingtai Mother and Children Care Courtyard,Xingtai 054000,Chin)
出处
《现代药物与临床》
CAS
2018年第7期1566-1572,共7页
Drugs & Clinic
关键词
梓醇
顺铂
卵巢颗粒细胞
凋亡
保护作用
catalpol
cisplatin
ovarian granulosa ceils
apoptosis
protection
