摘要
目的研究散结灵胶囊治疗子宫肌瘤的效应机制。方法采用长期外源性雌激素、孕激素肌肉注射复制大鼠子宫肌瘤模型;采用肾上腺素法复制大鼠急性血瘀模型;采用醋酸扭体法复制小鼠疼痛模型,综合评价散结灵胶囊治疗子宫肌瘤的药效学作用特点和效应机制。结果散结灵胶囊可显著降低子宫肌瘤模型大鼠子宫和卵巢脏器系数,下调血清中雌二醇(E2)和催乳素(PRL)水平,改善子宫肌纤维明显的增生肥大、肌层增厚、肌纤维排列紊乱、内膜腺体数量增多、中性粒细胞浸润等病理改变;明显改善急性血瘀模型大鼠全血的低切变率,延长凝血酶原时间(PT),降低血浆中的纤维蛋白原(FIB)含有量;显著抑制醋酸致小鼠腹腔疼痛刺激的扭体反应潜伏期和扭体次数。结论散结灵胶囊对鼠类具有较好的改善子宫肌瘤病变、活血化瘀、镇痛作用。
AIM To evaluate the efficacy and mechanism of Sanjieling Capsules on uterine fibroids.METHODS The rat models of uterine fibroids induced by long-term intramuscular injection of artificial estrogen and progesterone,as well as epinephrine-based rat models of acute blood stasis and acetic acid-based mouse models of pain were prepared for a comprehensive evaluation of the pharmacodynamic effect and mechanism of Sanjieling Capsules in the treatment of uterine fibroids. RESULTS The intervention of Sanjieling Capsules resulted in significantly reduced uterus and ovary coefficient,decreased serum levels of E2 and PRL,and obviously improved uterine myofibrotic hyperplasia,thickening of muscular layer,adenomyosis,overly developed endometrial glands,and infiltration of neutrophils; significantly improved low shear rate of whole blood,prolonged prothrombin time（ PT）,and decreased plasmatic fibrinogen content（ FIB） in rat models of acute blood stasis. The latency and frequency of torsional response to acetic acid-induced abdominal pain were significantly inhibited in mice.CONCLUSION Sanjieling Capsules can effectively manage rodents＇ uterine leiomyoma,blood stasis and pain.
作者
刘丹
陈霞
岳枫
陈会丛
黄倩
张晓笛
张瀚涛
李晋生
杜莹洁
LIU Dan;CHEN Xia;YUE Feng;CHEN Hui-cong;HUANG Qian;ZHANG Xiao-di;ZHANG Han-tao;LI Jin-sheng;DU Ying-jie(National Engineering Research Center for R＆D of TCM Multi-ingredient Drugs,Beijing Zhongyan Tongrentang Medicine R＆D Co.,Ltd.,Beijing 100079,China)
出处
《中成药》
CAS
CSCD
北大核心
2018年第8期1676-1681,共6页
Chinese Traditional Patent Medicine
关键词
散结灵胶囊
子宫肌瘤
效应机制
Sanjieling Capsules
uterine fibroids
efficacy and mechanism
