摘要
目的优化表面活性剂-超声波协同提取石榴瓤总黄酮工艺,并考察其动力学、热力学行为。方法在单因素试验基础上,以总黄酮得率为评价指标,十二烷基硫酸钠(SDS)用量、乙醇体积分数、超声温度为影响因素,中心组合设计优化提取工艺。然后,考察提取过程中的动力学、热力学行为。结果最佳条件为SDS用量0.84%,乙醇体积分数61%,超声温度53℃,总黄酮得率1.736%。加入表面活性剂(SDS)后,提取速率常数(k)、表面扩散系数(DS)显著增加,提取为吸热熵增加的自发过程。结论该方法稳定可靠,可用于表面活性剂-超声波协同提取石榴瓤总黄酮。
AIM To optimize the surfactant-ultrasonic co-extraction for total flavonoids from Punica granatum fleshes and to investigate the kinetic,thermodynamic behaviors. METHODS With total flavonoids yield as an evaluation index,sodium dodecyl sulfate（ SDS） consumption,ethanol concentration and ultrasonic temperature as influencing factors,the extraction was optimized by central composite design on the basis of single factor test. Then kinetic,thermodynamic behaviors during the extraction were investigated. RESULTS The optimal conditions were determined to be 0. 84% for SDS consumption,61% for ethanol concentration,and 53 ℃ for ultrasonic temperature,the total flavonoids yield was 1. 736%. After the addition of surfactant（ SDS）,significantly increased extraction rate constant（ k） and surface diffusion coefficient（ DS） were observable,and the extraction was a spontaneous process with increased endothermic entropy. CONCLUSION This stable and reliable method can be used for the surfactant-ultrasonic co-extraction for total flavonoids from P. granatum fleshes.
作者
王占一
王京龙
王玉海
戴博
郑丹丹
毕海丹
WANG Zhan-yi;WANG Jing-long;WANG Yu-hai;DAI Bo;ZHENG Dan-dan;BI Hal-dan(College of Food Science and Phaumaceutical Engineering,Zaozhuang University,Zaozhuang 277160,China;Department of Pharmacy,Air Force General Hospital of PLA,Beijing 100142,China)
出处
《中成药》
CAS
CSCD
北大核心
2018年第8期1726-1732,共7页
Chinese Traditional Patent Medicine
基金
国家星火计划项目(2012GA740049
2015GA740027)
山东省自然科学基金(ZR2013BL018)
山东省重点研发计划项目(2016GSF202010)
关键词
石榴
瓤
总黄酮
表面活性剂-超声波协同提取
中心组合设计
动力学行为
热力学行为
Punica granatum L.
fleshes
total flavonoids
surfactant-ultrasonic co-extraction
central composite design
kinetic behaviors
thermodynamic behaviors
