摘要
目的观察医用臭氧对博来霉素致肺纤维化大鼠血清及支气管肺泡灌洗液中TNF-ɑ、TGF-β1水平及肺功能的影响。方法于2016年8月—2017年1月选取由郑州大学动物实验中心提供的40只清洁级雄性SD大鼠,随机分为对照组、模型组、激素组及试验组各10只,其中对照组采用气管内滴入生理盐水,其余大鼠均采用气管内滴入博来霉素制作肺纤维化模型,每天给予生理盐水(对照组和模型组)、地塞米松(激素组)、医用臭氧(试验组)腹腔注射,全部大鼠第28天测定肺功能后处死,肺组织切片HE染色观察肺泡炎和肺纤维化改变程度,ELISA法测定血清及肺泡灌洗液中TNF-ɑ、TGF-β1的含量。结果 28 d时试验组肺功能指标FEV0.3、FEV0.3/FVC、PEF分别为(5.03±0.48)m L、(60.93±1.81)%、(34.32±1.63)m L/L,模型组分别为(3.36±0.33)m L、(42.98±1.91)%、(21.90±1.56)m L/L,激素组分别为(4.26±0.35)m L、(50.38±2.04)%、(26.95±1.21)m L/L,对照组分别为(5.90±0.52)m L、(78.15±2.11)%、(38.82±1.44)m L/L,试验组分别与模型组、激素组、对照组比较差异有统计学意义(P<0.05)。试验组肺纤维化病理评分(1.40±0.52)分低于模型组(2.80±0.42)分与激素组(2.10±0.74)分,差异有统计学意义(P<0.05);血清和BALF中TNF-α水平分别为试验组(12.03±1.44)、(1.75±0.53)pg/m L、模型组(28.58±1.48)、(2.89±0.13)pg/m L、激素组(18.14±1.67)、(1.95±0.72)pg/m L、对照组(10.07±0.64)、(1.50±0.03)pg/m L,血清和BALF中TGF-β1分别为试验组(75.41±1.93)、(15.10±0.74)pg/m L、模型组(96.11±1.56)、(23.13±1.45)pg/m L、激素组(85.06±1.97)、(17.98±0.79)pg/m L、对照组(62.91±1.16)、(11.97±0.94)pg/ml,试验组与模型组、激素组、对照组比较差异有统计学意义(P<0.05)。结论医用臭氧能下调大鼠血清及BALF中TNF-ɑ、TGF-β1的水平,降低肺泡炎及肺纤维化程度,延缓大鼠肺纤维化病程。
Objective To observe the effects of medical ozone on TNF-α, TGF-β1 levels and lung function in serum and bronchoalveolar lavage fluid in rats with bleomycin-induced pulmonary fibrosis. Methods A total of 40 clean male SD rats provided by Animal Experimental Center of Zhengzhou University from August 2016 to January 2017 were selected. They were randomly divided into control group, model group, hormone group and experimental group each with10 rats. The control group received intratracheal drops. Into normal saline, the rest of the rats were intubated with bleomycin in the lung fibrosis model, given daily physiological saline（control group and model group）, dexamethasone（hormone group）, medical ozone（test group） abdominal cavity after injection, all rats were dead on the 28 th day after the determination of lung function. Lung tissue sections were stained with HE to observe the changes of alveolitis and pulmonary fibrosis. Serum and bronchoalveolar lavage fluid were measured for TNF-α and TGF-β1 by ELISA. Results On the 28 th day, FEV0.3, FEV0.3/FVC and PEF in the experimental group were（5.03 ±0.48）mL,（60.93 ±1.81）%,（34.32±1.63）mL/L, respectively. The model group was（3.36±0.33）mL,（42.98±1.91）%,（21.90±1.56）mL/L, hormone group（4.26 ±0.35）mL,（50.38 ±2.04）%,（26.95 ±1.21）mL/L, respectively. The control group of（5.90 ±0.52）mL,（78.15 ±2.11）%,（38.82 ±1.44）mL/L, and the difference between the experimental group and model group, hormone group and control group were statistically significant（P〈0.05）. The pathological score of pulmonary fibrosis in the experimental group was（1.40±0.52）points lower than that in the model group（2.80 ±0.42）points and the hormone group（2.10 ±0.74）points（P〈0.05）; the levels of TNF-α in the serum and BALF were respectively For the test group（12.03±1.44）,（1.75±0.53）pg/mL,model group（28.58 ±1.48）,（2.89 ±0.13）pg/mL, hormone group（18.14±1.67）,（1.95 ±0.72） pg/mL, control group（10.07±0.64）,（1.50±0.03） pg/mL, TGF-β1 in serum and BALF were respectively the test group（75.41±1.93）,（15.10±0.74） pg/mL, model group（96.11±1.56）,（23.13±1.45）pg/mL, In the hormone group（85.06±1.97）,（17.98±0.79）pg/mL, control group（62.91 ±1.16）,（11.97 ±0.94）pg/mL, the difference between the experimental group and the model group, the hormone group, and the control group was statistically significant（P〈0.05）. Conclusion Medical ozone can down-regulate the levels of TNF-α and TGF-β1 in rat serum and BALF, reduce alveolitis and pulmonary fibrosis,and delay the progression of pulmonary fibrosis in rats.
作者
李珂
张筠
张铁栓
LI Ke;ZHANG Yun;ZHANG Tie-shuan(The Second Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan Province, 450000 China)
出处
《系统医学》
2018年第12期1-3,11,共4页
Systems Medicine
