摘要
目的建立HPLC法测定麝香接骨丸羟基红花黄色素A含量方法。方法色谱柱:Agilent Extend C18色谱柱(250 mm×4.6 mm,5μm);流动相:甲醇-0.7%磷酸溶液(30:70);流速:1.0 m L/min;检测波长:403 nm;柱温:30℃;进样量:10μL。结果羟基红花黄色素A的回归方程为Y=25 981X-20 503(r=0.999 7),线性范围为3.20~102.27μg/m L。平均加样回收率97.95%,RSD为2.87%(n=6)。结论该法操作简便,准确,重现性好,可作为本制剂的质量控制标准。
Objective This paper tries to establish a HPLC method for the determination of hydroxysafflor yellow A content in Huoxiang Jiegu Pills. Methods Column： Agilent Extend C18 column（250 mm ×4.6 mm, 5 μm）; mobile phase： methanol-0.7% phosphoric acid solution（30：70）; flow rate： 1.0 mL/min; detection wavelength： 403 nm; column temperature： 30 ℃; injection volume： 10 μL. Results The regression equation of hydroxysafflor yellow A was Y =25981 X-20 503（r=0.9997）, and the linear range was 3.20 to 102.27 μg/mL. The average recovery rate was 97.95%, and the RSD was 2.87%（n=6）. Conclusion This method is simple, accurate and reproducible, and can be used as the quality control standard of the preparation.
作者
戴作波
姜志戎
DAI Zuo-bo;JIANG Zhi-rong(Department of Phamlaey, Baoying County Traditional Chinese Medicine Hospital, Baoying, Jiangsu Province, 225800 Chin)
出处
《系统医学》
2018年第12期156-159,共4页
Systems Medicine
关键词
麝香接骨丸
基红花黄色素A
含量测定
HPLC法
Shexiang Jiegu Pill
Baieale yellow pigment A
Content determination
HPLC method
