摘要
目的 探讨膀胱移行细胞癌TGFβ1蛋白及mRNA异常表达的临床意义。 方法 采用SP法检测 74例膀胱癌组织中TGFβ1蛋白的表达 ;QRT PCR方法检测 43例膀胱癌TGFβ1mRNA转录水平。 结果 膀胱癌TGFβ1蛋白表达阳性率为 89.2 % ,T2 ～T4 浸润性膀胱癌TGFβ1过表达率为 83.8% ,明显高于Ta～T1表浅性膀胱癌 (33.3% ) ,P <0 .0 5 ;复发转移组 30例中TGFβ1过表达 2 9例 ,而无瘤存活组 44例中过表达 30例 ,P <0 .0 0 5 ;膀胱癌组织TGFβ1mRNA含量明显高于正常膀胱组织。 结论 TGFβ1过表达与膀胱癌浸润生长方式和预后等有密切关系 ,可能成为评价膀胱癌预后的有效指标之一。
Objective To investigate the influence of the aberrant protein expression and mRNA transcription of transforming growth factor β 1(TGFβ 1) on the biological behavior of human bladder transitional cell carcinomas. Methods The expression of TGFβ 1 was investigated in 74 specimens of TCCs by SP immunohistochemistry staining, and the level of TGFβ 1 mRNA were determined in 43 cases of TCCs by the method of quantitative RT PCR. Results The positives expression rate of TGFβ 1 was 89.2%. Superficial tumors had lower overexpressive rate of TGFβ 1(33.3%) than the invasive TCCs(83.8%), P <0.05. Significantly higher overexpressive rate of TGFβ 1 was observed in recurrent and metastatic TCCs(29/30) than the tumor free survival TCCs(30/44), P <0.005. The level of TGFβ 1 mRNA expression in TCCs was higher than that detected in normal bladder epithelium, P <0.05. Conclusions The expression of TGFβ 1 in TCCs is higher than in normal bladder tissues. The overexpression of TGFβ 1 is related significantly with the invasive tumors and with the recurrence and the 5 years survival rate of TCCs. Overexpression of TGFβ 1 could be one of the effective prognostic markers for patients with TCCs.
出处
《中华泌尿外科杂志》
CAS
CSCD
北大核心
2002年第8期498-500,共3页
Chinese Journal of Urology
关键词
过表达
膀胱肿瘤
转化生长因子Β1
TGFΒ1
Bladder neoplasms
Carcinoma,transitional cell
Transforming growth factor β 1
