摘要
利用差分脉冲伏安法对芹菜总黄酮进行定量分析。选择芦丁纯品作为测定芹菜总黄酮的标准品,以纯玻碳电极为工作电极,首先采用循环伏安法优化测定条件,然后在最佳测定条件下,采用差分脉冲伏安法测定芹菜中总黄酮的含量。试验表明,芹菜总黄酮氧化峰电流与其浓度在2.0×10-6 mol/L^1.3×10^(-4) mol/L范围内呈良好的线性关系,检出限达到1.2×10^(-11) mol/L(S/N=3)。
To create a quantitative analysis method of differential pulse voltammetry to determine the concentration of total flavonoids in celery. The pure rutin was chosen as a standard product,and the glassy carbon electrode was used as the working electrode,the conditions of the flavonoids were optimized by cyclic voltammetry,then differential pulse voltammetry was used to determine its concentration in celery. The experiment results indicated that the oxidation peak current of the flavonoids in celery were linear with its concentration in the range of 2.0×10-6 mol/L-1.3×10-4 mol/L with a detection limit（S/N=3）of 1.2×10-11 mol/L.
作者
赖红伟
舒适
薛梦蝶
李玲
刘宇博
兰月娇
袁园
廉国乾
刘荣兰
毛蔚荻
李婷毓
曹宏梅
LAI Hong-wei, SHU Shi, XUE Meng-die, LI Ling, LIU Yu-bo, LAN Yue-jiao, YUAN Yuan, LIAN Guo-qian, LIU Rong-lan, MAO Wei-di, LI Ting-yu, CAO Hong-mei(Jilin Medical College, Jilin 132013, Jilin, Chin)
出处
《食品研究与开发》
CAS
北大核心
2018年第16期130-133,共4页
Food Research and Development
基金
吉林医药学院大学生创新创业训练计划项目(2016B316
201743
201613706017
201715)
吉林省中医药科技项目(2017102)
吉林省卫生计生青年科技骨干培养计划项目(2016Q054)
吉林省卫生计生科技创新自筹经费项目(2017ZC034)
2017年吉林市青年科技工作者创新创业大奖赛项目(201750252)
关键词
电化学法
芹菜
总黄酮
循环伏安法
差分脉冲伏安法
electrochemical method
celery
total flavonoids
cyclic voltammetry（CV）
differential pulse voltammetry （DPV）
