摘要
观察铁皮石斛复方对＂饮食不节＂致高血压大鼠的药效及其相关机制。50只SD大鼠用高糖高脂饲料复合饮酒连续喂养4周,造模后根据血压随机分为模型组、缬沙坦组（8 mg·kg^（-1））、铁皮石斛复方3个剂量组（1.65,3.30,5.00 g·kg^（-1））,每组10只,另取10只SD大鼠作为正常组。连续给药4周,测定大鼠的血压;眼眶取血,测定血清胆固醇（TC）、甘油三酯（TG）、低密度脂蛋白胆固醇（LDL-c）、高密度脂蛋白胆固醇（HDL-c）,计算动脉粥样硬化指数（AI）;硝酸还原酶法检测血清一氧化氮（NO）;酶联免疫法（ELISA）测定血清内皮素-1（ET-1）、细胞间黏附分子-1（ICAM-1）;末次给药后处死大鼠,Western blot法检测各组大鼠胸主动脉中PI3K/AKT/e NOS蛋白的表达情况;分离主动脉,HE染色,观察胸主动脉内皮和血管结构的变化;Masson染色观察主动脉胶原沉积情况;免疫组化法观察主动脉内皮型一氧化氮合酶（e NOS）和ICAM-1的表达情况。结果显示,铁皮石斛复方能够明显降低高血压大鼠的血压,升高HDL-c,降低AI,同时能够升高血清中NO含量,降低ET-1和ICAM-1的水平,促进PI3K/AKT/e NOS蛋白表达。铁皮石斛复方组病变程度减轻,胶原沉积明显减少。同时铁皮石斛复方能够明显增加e NOS的表达,降低ICAM-1的生成。铁皮石斛复方对于＂饮食不节＂致高血压大鼠具有明显的抗高血压作用,其机制可能与改善血脂水平,降低AI,增强PI3K/AKT/e NOS信号通路,增加NO生成,抑制ICAM-1,ET-1分泌,保护血管内皮,改善主动脉病变等有关。
To observe the efficacy of compound Dendrobium on Sprague Dawley rats（ SD） hypertension model induced by ＂ dietary disorders＂ and its relevant mechanism,totally 50 SD rats were fed with high-sugar,high-fat diet and alcohol for four weeks. According to the blood pressure after modeling,the rats were divided into model group,valsartan group（ 8 mg·kg^（-1））,low,medium and highdose Dendrobium candidum compound groups（ 1. 65,3. 30,5. 00 g·kg^（-1））,with 10 rats in each group,and the other 10 SD rats were also taken as the normal group. After four weeks of treatment,blood pressure was measured. Orbital blood was collected for the determination of serum cholesterol（ TC）,triglycerides（ TG）,low-density lipoprotein cholesterol（ LDL-c）,high-density lipoprotein cholesterol（ HDL）,calculation of atherosclerosis index（ AI）. Nitric acid reductase method was used to detect serum nitric oxide（ NO）; the levels of serum endothelin-1（ ET-1） and intercellular adhesion molecule-1（ ICAM-1） were measured by ELISA. The rats were put to death after the last administration,and the protein expressions of PI3 K/AKT/e NOS in thoracic aorta of rats in each group were detected by Western blot. The aorta was separated and stained with hematoxylin-eosin（ HE） to observe the changes in the endothelium and blood vessels in the thoracic aorta. Masson staining was used to observe the formation of aortic collagen. The expressions of nitric oxide synthase（ e NOS） and ICAM-1 in aortic endothelial cells were observed by immunohistochemistry. In contrast,the results show D. candidum compound can significantly reduce the blood pressure in hypertensive rats,increase HDL-c,and reduce AI,while increasing serum NO content,decreasing ET-1 and ICAM-1 levels and promoting PI3 K/AKT/NOS protein expressions. The lesion degree of the D. candidum compound group was reduced,and the collagen deposition was significantly reduced. Meanwhile,D. candidum compound can significantly increase the expression of e NOS,and reduce the formation of ICAM-1. Therefore,D. candidum compound has an obvious antihypertensive effect on hypertensive rats,which may be related to the increase in PI3 K/AKT/e NOS signaling pathways and NO generation,the inhibition of the secretion of ICAM-1 and ET-1,the protection of the vascular endothelium and the improvement of aortic disease.
作者
尹超
徐俞悦
陈国杨
李波
何茂
施秋秋
王婷
吕圭源
陈素红
YIN Chao;XU Yu-yue;CHEN Guo-yang;LI Bo;HE Mao;SHI Qiu-qiu;WANG Ting;LYU Gui-yuan;CHEN Su-hong(Wenzhou Medical College,Wenzhou 325035,China;Zhejiang Chinese Medical University,Hangzhou 310053,China;Zhejiang Technology University,Hangzhou 310014,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2018年第11期2345-2351,共7页
China Journal of Chinese Materia Medica
基金
国家重点研发计划项目(2017YFC1702202,2017YFC1702203)
国家自然科学基金面上项目(81673638)
国家自然科学基金青年基金项目(81703772)
浙江省自然科学基金项目(LQ17H280005)
关键词
铁皮石斛复方
高血压
饮食不节
动脉粥样硬化指数
血管内皮因子
Dendrobium candidum compound
hypertension
irregular diet
atherosclerosis index
vascular endothelial factor
