摘要
从长期受到有机磷污染的土壤里分离一株高效降解有机磷的假单胞菌MET75(pseudomonas.MET75).将MET75菌悬液(OD_(600 nm)=1.0)以2%的接种量加入含有乐果的无机盐培养基中,摇瓶培养120 h,菌株对50 mg/L乐果的降解率达89.66%.高效液相色谱(HPLC)结果显示,MET75在降解乐果过程中无新的中间物质峰检出,初步预测降解是完全矿化作用.研究还发现,MET75在pH 7.0 11.0范围内对卵磷脂也有较强的水解能力,将MET75菌液与土壤混合,自然环境下作用72 h后,土壤速效磷含量由8.42 mg/kg升至16.84 mg/kg;小白菜盆栽实验结果显示,实验组小白菜较对照组:鲜重增加了34.63%,株高增加了12.43%,根长增加了24.36%,茎粗增加了51.79%.综上所述,MET75对磷化工污水的处理和生物农业的发展均具有重要意义.
A strain MET75 was isolated from soil contaminated by organophosphorus for a long time andwas identified as Pseudomonas sp. The MET75 bacterial suspension （OD600 nm = 1. 0） was added to the MSMmedium at 2% inoculums, the degradation rate of the strain to 50 mg / L dimethoate was 89. 66% after 120 hculture in shake flask. High-performance liquid chromatography （HPLC） results showed that there were nonew intermediate peaks in the process of MET75 degrade dimethoate and the degradation is completelymineralized. We also found that MET75 also has strong hydrolytic capacity for lecithin in the range of pH7. 0-11. 0. Mixing MET75 with soil for 72 h, the soil available P content increased from 8. 42 mg / kg to 16.84. The results of potted cabbage experiment showed that compared with the control group, the fresh weight ofcabbage in experimental group increased by 34. 63%, the plant height increased by 12. 43%, the root lengthincreased by 24. 36% and the stem diameter increased by 51. 79%. In summary, MET75 have greatimportance on phosphorus chemical wastewater treatment and the development of biological agriculture.
作者
刘诚
张钲
佘梦林
唐梦君
倪红
LIU Cheng;ZHANG Zheng;SHE Menglin;TANG Mengjun;NI Hong(Hubei Collaborative Innovation Center for Green Transformation of Bio-resources,College of Life Sciences,Hubei University,Wuhan 430062,China)
出处
《湖北大学学报（自然科学版）》
CAS
2018年第5期457-461,469,共6页
Journal of Hubei University：Natural Science
基金
湖北省科技厅公益性科技计划项目(2014BCB030)资助
