摘要
通过生物信息学方法,对毛竹(Phyllostachys edulis(Carrière)J.Houzeau)TPS基因家族的成员进行鉴定,并对其编码蛋白的理化性质、基因结构、进化关系、蛋白结构、启动子元件及表达模式进行了分析。结果表明,毛竹全基因组含有14个TPS候选基因,大小为693~2439 bp。编码蛋白等电点为5.08~8.17。系统发育分析结果显示,毛竹含有TPS-a、TPS-b、TPS-e/f、和TPS-g 4个亚家族,成员数目分别为6、5、2、1个。TPS蛋白质二级结构中,α-螺旋和无规则卷曲所占比重较大;毛竹TPS基因家族各成员蛋白三维结构比较相似。基因启动子分析共获得50个调控元件,可分为6大类,其中光响应相关元件数量最多,共包含17个顺式调控元件。基于转录组测序数据构建的基因表达谱热图分析结果表明,Pe TPS在叶、花和笋等7个组织中的表达差异明显,表现出组织特异性,其中Pe TPS9仅在早花期花序中表达,Pe TPS8仅在叶中表达。
To explore the characteristics and evolutionary relationships of the TPS family in Phyllostachys edulis, we identified the TPS genes in P. edulis and analyzed their physicochemical properties,gene structure,evolutionary relationship,protein secondary and tertiary structure, promoter elements, and expression patterns based on bioinformatics methods. A total of 14 TPS genes were identified in P. edulis,which ranged in size from 693 to2439 bp. The encoding protein isoelectric points ranged from 5. 08 to 8. 17. Phylogenetic analysis suggested that the TPS family members from P. edulis could be divided into four subfamilies（a,b,e/f,g）,with considerably different gene structures. The α-helix and random coil components in these proteins were dominant elements,and the predicted tertiary structure of the proteins in the Pe TPS gene family were similar. We identified 50 cis-acting regulatory elements through promotor analyses, which were classified into six categories according to their function. The heatmap of gene expression based on the RNA-seq datarevealed that the Pe TPS genes were expressed differently among seven different tissues,including leaves,flowers,and shoots,and thus exhibited tissue-specific expression patterns.Pe TPS9 was only expressed in the early panicle and Pe TPS8 was only expressed in the leaves.This research provides a theoretical foundation for deeper analysis of the function of the TPS genes in P. edulis.
作者
秦政
郑永杰
张文根
张龙
黎祖尧
杨光耀
Qin Zheng;Zheng Yong-Jie;Zhang Wen-Gen;Zhang Long;Li Zu-Yao;Yang Guang-Yao(Jiangxi Agricultural University,Jiangxi Provincial Key Laboratory Germplasm Resources and Utilization,Nanchang 330045,China;Jiangxi Academy of Forestry,Nanchang 330032,China;The 2011 Collaborative Innovation Center of Jiangxi Typical Trees Cultivation and Utilization,Nanchang 330045,China)
出处
《植物科学学报》
CAS
CSCD
北大核心
2018年第4期575-585,共11页
Plant Science Journal
基金
国家科技支撑计划项目(2015BAD04B01)
关键词
毛竹
萜类合成酶
进化分析
表达模式
Phyllostachys edulis
Terpene synthase
Phylogenetic analysis
Expression pat-tern analysis
