摘要
【目的】筛选出提高无核葡萄果实中可溶性糖含量的优良砧木,为葡萄果实品质调控提供理论依据。【方法】以7种不同砧木嫁接的4年生克瑞森无核葡萄和自根苗为试材,利用HPLC技术测定果实发育过程中葡萄糖、果糖和蔗糖含量,测定糖代谢相关酶活性,分析不同砧木对克瑞森无核葡萄果实中糖分积累和蔗糖代谢相关酶活性的动态变化影响,及二者间的相关性变化。【结果】果实发育过程中克瑞森无核葡萄砧木嫁接苗和自根苗的果实糖分积累主要以葡萄糖和果糖为主,蔗糖为辅。砧木嫁接在不同程度上能够提高克瑞森无核葡萄的可溶性糖的积累,不同砧木之间存在较大差异。5BB、5C、101-14MG和SO4嫁接能够显著提高果实内蔗糖、葡萄糖、果糖和总糖含量,其中101-14MG嫁接苗可溶性糖与自根苗比最大提高了14.67%,促进作用最明显。而贝达和188-08不利于提高果实内糖含量。在果实发育期间各砧木嫁接苗果实蔗糖转化酶(AI和NI)呈先升高后降低的趋势,在发育中期(花后75 d)酶活性达到峰值;而蔗糖合成酶整体呈上升趋势,蔗糖磷酸合成酶(SPS)呈先升后降再升的趋势。与自根苗相比,7个砧木中101-14MG更利于提高克瑞森无核葡萄的AI和SS-c活性,SO4更利于提高其SS-c和SPS活性,嫁接苗果实内糖组分含量与AI、SPS和SS-s活性相关性较大,其共同作用影响了可溶性糖含量的变化,其中SS-s对糖分的积累贡献最大。【结论】7种砧木嫁接的克瑞森无核葡萄中,SO4、101-14MG、5BB和5C嫁接均能够显著提高果实内可溶性糖的积累,其中101-14MG表现最佳。克瑞森无核嫁接苗果实内可溶性糖的积累是AI、SPS和SS-s活性共同调控的结果,其中SS-s是克瑞森无核葡萄果实糖积累中最重要的调控因子。
【Objective】This project aims to select an excellent rootstock with soluble sugar content in Crimson seedless grape fruit in the hope of providing the theoretical basis for fruit quality regulation.【Method】The contents of glucose,fructose and sucrose in fruit development were measured by HPLC technique with 7 different rootstock and 4 year old Crimson seedless grapes and self-rooted seedlings,and the activity of glucose metabolism related enzymes was measured,and the effects of different rootstocks on the dynamic changes of sugar accumulation and sucrose metabolization-related enzyme activity in the seedless grape fruit of Crimson and the correlation between them were studied.【Result】In the fruit development process,the sugar accumulation of Crimson seedless grape rootstock grafted seedling and self root was mainly glucose and fructose,and sucrose was supplemented. Rootstock grafting can improve the soluble sugar accumulation of crimson seedless grape to different degrees,and there was great difference between the different rootstocks,5BB,5C,101-14MG and SO4 grafting could significantly increase sucrose,glucose,fructose and total sugar in fruits.The ratio of soluble sugar to 101-14MG was increased by 14. 67% and the promotion effect was most obvious. However,beta and 188-08 could not improve the sugar content in fruits. In the middle of development（after 75 d）,the activity of the enzyme reached its peak,while the sucrose synthase was ascending and the sucrose phosphate synthase（SPS） was ascending and then rising. Compared with the self root,the 101-14MG of the 7 rootstocks was more beneficial to improve the AI and SS-c activity of the Crimson seedless grape,the SO4 was more beneficial to improve its SS-c and SPS activity,and the content of sugar component in grafted seedlings was more correlated with AI,SPS and SS-s activity. The interaction affected the change of soluble sugar content,among which,SS-s contributed the most to the accumulation of sugar. 【Conclusion】The grafts of SO4,（101-14） MG.（5BB） and 5C grafted with 7 rootstocks grafted in Crimson seedless grapes can significantly increase the accumulation of soluble sugar in fruit,among which,101-14MG performance is the best. The accumulation of soluble sugar in Crimson seedlings is the result of the co-regulation of AI,SPS and SS-s activities,and among them,the SS-s is the most important regulating factor in sugar accumulation of Crimson seedless grape fruit.
作者
钟海霞
张付春
周晓明
潘明启
张雯
谢辉
韩守安
艾尔买克.才卡斯木
伍新宇
ZHONG Hai-xia;ZHANG Fu-chun;ZHOU Xiao-ruing;PAN Ming-qi;ZHANG Wen;XIE Hui;HAN Shou-an;Ermek Caiksimu;WU Xin-yu(Institute of Horticultural Crops,Xinjiang Academy of Agricultural Scicnces/Scientific Observing arid Experimental Station of Pomology（Xinjiang）,Ministry of Agriculture,Urumqi 830091,China)
出处
《新疆农业科学》
CAS
CSCD
北大核心
2018年第6期1002-1010,共9页
Xinjiang Agricultural Sciences
基金
新疆维吾尔自治区自然科学基金项目(2016D01B041)~~
关键词
无核葡萄
砧木
可溶性糖含量
酶活性
Seedless grape
rootstock
soluble sugar content
enzyme activity
