摘要
目的:建立神香草抗哮喘有效部位的定性定量控制方法。方法:采用高效液相色谱法测定神香草有效部位中的迷迭香酸,并建立特征图谱;采用紫外分光光度法测定神香草有效部位中的总黄酮和总多糖。结果:神香草抗哮喘有效部位中迷迭香酸含量为3.68%,总黄酮含量为35.10%,总多糖含量为17.12%,建立了以5个共有峰为指标成分的神香草有效部位HPLC特征图谱。结论:本研究所建立的定性定量控制方法,能全面评价神香草抗哮喘有效部位的质量,并可为研究新药奠定基础。
Objective：To establish a qualitative and quantitative control method for the effective parts of Hyssopus cuspidatus Boriss anti-asthmatic.Methods：An HPLC was used to measure the contents of rosmarinic acid in the effective parts of Hyssopus cuspidatus Boriss anti-asthmatic and the HPLC specific chromatogram was also established.UV was used to measure the contents of total flavonoids and total polysaccharide of the effective parts ofHyssopus cuspidatus Boriss anti-asthmatic.Results：The contents of rosmarinic acid,total flavonoids and total polysaccharide were 3.68%,35.10% and 17.12%,respectively;HPLC specific chromatogram consisted of five common peaks.Conclusion：The method established in this study can be applied in the quality control for qualitative study on and quantitative stady on the effective parts of Hyssopus cuspidatus Boriss anti-asthmatic and can lay a foundation for the study of new drugs.
作者
戎晓娟
康雨彤
袁凤娟
贺金华
李红英
RONG Xiao-juan;KANG Yu-tong;YUAN Feng-juan;HE Jin-nua;LI Hong-ying(Xinjiang Institute of Material Mediea,Urumqi 830004,China;Xinjiang Uygur Autonomous Region for Food and Drug Control,Urumqi 830002,China;The Chinese People＇s Liberation Army Corps,Sichuan,Chengdu 610041,China)
出处
《药物分析杂志》
CAS
CSCD
北大核心
2018年第8期1437-1441,共5页
Chinese Journal of Pharmaceutical Analysis
基金
国家自然科学基金项目(81560644)
新疆维吾尔自治区公益性科研院所基本科研业务经费资助项目(KYGY2016167)
关键词
神香草
抗哮喘
质量控制
迷迭香酸
特征图谱
总黄酮
总多糖
Hyssopus cuspidatus Boriss
anti-asthma
quality control
rosmarinic acid
specific chromatogram
total flavonoids
total polysaccharide
