摘要
研究了大豆膳食纤维对糖尿病大鼠胰腺氧化性损伤的修复效果。腹腔注射链脲佐菌素溶液(150 mg/kg)构建糖尿病大鼠模型,连续灌胃大豆膳食纤维42 d,测定各组大鼠体重、胰腺体比、血清中谷胱甘肽过氧化物酶(Glutathione peroxidase,GSH-Px)、超氧化物歧化酶(Superoxide dismutase,SOD)及丙二醛(Malonic dialdehyde,MDA)含量,并取胰腺组织制作H&E染色病理学切片进行观察。与模型组大鼠相比,各灌胃组大鼠体重均较模型组均显著升高(P<0.05);各灌胃组大鼠胰腺体比均较模型组大鼠不同程度下降,中、高剂量组大鼠脏器比较模型组大鼠下降显著(P<0.05);除低剂量大豆膳食纤维组外,各灌胃组大鼠血清GSH-Px及SOD含量均较模型组大鼠不同程度上升(P<0.01),血清MDA含量均不同程度下降(P<0.01),且由胰腺组织切片观察得知,各灌胃组大鼠胰腺组织损伤程度明显减轻,胰岛细胞数目增多,且细胞水肿变形程度减轻。结果表明,大豆膳食纤维可有效降低并修复糖尿病对大鼠的氧化损伤程度。
The thesis investigated the effect of different dietary soybean dietary fiber on pancreatic oxidative damage repair of diabetic rats. Diabetic rat model was constructed by intraperitoneal injection of alloxan solution （ 150 mg/kg） , after modeling is successful, the body and pancreas weight, the levels of serum, Glutathione peroxidase （GSH Px）, Superoxide dismutase （SOD） and Malonic dialdehyde （MDA） were measured after 42 days of continu ous administration of soybean dietary fiber, and stained with pancreatic tissue to prepare H＆E pathology sections were el group （P 〈0. O1 ）, serum MDA content decreased in varying degrees （P 〈0.0! ）. Histopathological examination of pancreas showed that the degree of pancreatic tissue injury was significantly reduced, the number of islet cells increased, and the degree of edema of cell edema was reduced. The results showed that soybean dietary fiber could repair oxidative damage and improve the diabetic rats on the pancreas in iury.
作者
佐兆杭
王颖
宫雪
刘淑婷
王迪
张东杰
张玉先
于寒松
曹龙奎
Zuo Zhaohang;Wang Ying;Gong Xue;Liu Shuting;Wang Di;Zhang Dongjie;Zhang Yuxian;Yu Hansong;Cao Longkui(Heilongjiang Bayi Agricultural University1,Daqing 163319;National Coarse Cereals Engineering Research Center2,Daqing 163319;College of Food Science and Engineering,Jilin Agricultural ＇ ~ 3 Umverslty,Changchun 130118)
出处
《中国粮油学报》
EI
CAS
CSCD
北大核心
2018年第8期13-18,共6页
Journal of the Chinese Cereals and Oils Association
基金
黑龙江省应用技术研究与开发计划重大项目(GA15B301)
黑龙江八一农垦大学研究生创新科研项目(YJSCX2017-Y57)
国家现代农业产业技术体系项目(CARS-04)
关键词
大豆膳食纤维
糖尿病
胰腺
氧化损伤
修复
soybean dietary fiber
diabetes
pancreas
oxidative damage
repair
