摘要
蒜头果为我国特有单种属植物,其果实富含神经酸,是一种很有开发前景的野生植物资源。为了解蒜头果中内生真菌的种类及分布情况,本研究对生长于裸露石灰岩山地和石灰岩林地两种生境的蒜头果分别取样,利用传统分离方法对蒜头果的根、树皮及叶组织中的内生真菌进行分离纯化,并依据形态学特征和r DNA-ITS序列对菌种进行鉴定。所有样品共分离获得112株内生真菌,分别属于10个属,其中紫霉属（Purpureocillium）、酵母样菌属（Acaromyces）、木霉属（Trichoderma）、淡紫紫孢菌（Purpureocillium lilacinum）为优势菌种。分析显示内生真菌在不同部位差异明显,根中内生真菌分布最为普遍,两种生境条件下分离率都为100%,对比之下树皮分离率分别为56.7%和63.3%,叶的都为26.7%。不同生境对蒜头果内生真菌具有明显影响,在裸露石灰岩山地根、树皮、叶的多样性指数分别为0.756、1.518和0.662,石灰岩林地分别为1.412、1.439和0.974。在两种生境条件下,树皮的真菌多样性相似,根和叶的多样性都表现出石灰岩林地高于裸露石灰岩山地。
Malania oleifera has a wild plant resource with huge prospective development, as their fruits abound in nervonic acids, and it is the special monotypic plant genera in China. To acquire the variety and distribution of endophytic fungi in Malania oleifera tree, we took the sample from two habitat of bare limestone mountain and limestone holt, respectively, isolated and purified the endophytic fungi from the roots, barks and leaves of Malania oleifera using the traditional separation methods, identificated these fungus according to their character of phenotype and r DNA-ITS. The research showed that there were 112 endophytic fungus from all the samples,belongs to 10 genus, and Purpureocillium, Acaromyces, Trichoderma is the domaint fungi genus, the preponderant strain is Purpureocillium lilacinum. The difference of separation rate of endophytic fungi in the different tissue of Malania oleifera is very obviously, the endophytic fungi in the root distributes the most widespread, the separation rate in the roots and leaves are both 100% in two habitat, and the rate of barks is 56.7%, 63.3%, respectively. The effect of different habitats on the endophytic fungi of Malania oleifera was apparently, the diversity index of roots,barks and leaves in the exposed limestone mountain was severally 0.756, 1.518, 0.662, and it was 1.412, 1.439 and0.974, respectively. The fungal diversity of barks were analogous in both habitats, and the diversity of roots and leaves in the limestone forest land both manifested higher than the bare limestone mountain.
作者
王毅
王娟
王四海
李云琴
原晓龙
陈中华
Wang Yi;Wang Juan;Wang Sihai;Li Yunqin;Yuan Xiaolong;Chen Zhonghua(The Key Laboratory of Forestry Plant Cultivation and Utilization,The Key Laboratory of Rare and Endangered Forest Plants of State Forestry Administration,Yunnan Academy of Forestry,Kunming,650204;State Key Laboratory of Phytochemistry and Sustainable Utilization of Plant Resources in Western China,Kunming Institute of Botany,Kunming,650201)
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2018年第9期3859-3866,共8页
Genomics and Applied Biology
基金
云南省科技计划项目(2015BB018)
植物化学与西部植物资源持续利用国家重点实验室开放课题(P2015-KF11)
国家科技基础性工作专项(2012FY110300)
国家自然科学基金资助项目(31560092)共同资助
关键词
蒜头果
植物内生真菌
多样性
石灰岩山地
Malania oleifera
Plant endophytic fungi
Diversity
Limestone mountain
