摘要
目前防控柑橘黄龙病的措施主要包括培育无病毒苗、防治木虱、铲除病树等。而多数防控措施的开展均需灵敏、可靠的早期诊断技术配合。综述了柑橘黄龙病的田间诊断方法以及基于电镜、血清学、高光谱、淀粉显色、常规PCR、巢式PCR、LAMP PCR、定量PCR、数字PCR的检测方法 ,并阐述了各自的优缺点。其中,定量PCR是目前较成熟和具有较高灵敏度的检测技术,可配合其他低成本检测方法对未显症、假阴性等样品进行早期检测;可实现单分子DNA绝对定量分析,数字PCR检测技术在柑橘黄龙病检测中也具有较好的应用前景。
At present, major measures against citrus Huanglongbing（HLB） includes planting of disease-free nursery stock,controlling psyllid vector by spraying pesticides and removal of infected trees. Most of these controlling mesures need sensitive and reliable early diagnosis technologies. This paper reviewed field diagnosis and detection methods for citrus Huanglongbing, associated with electron microscope, serology, hyperspectra, iodine-starch color development, conventional PCR, nested-PCR, LAMP PCR, real-time quantitative PCR and digital PCR, and evaluated their advantages and drawbacks. Among them, real-time quantitative PCR was relatively mature and sensitive detection technology currently,which can be used with other low-cost detection methods to conduct early detection for asymptomatic and false-negative samples. Besides, the digital PCR, which could realize absolute quantification of single molecule DNA, also had a good application prospect in the detection of citrus Huanglongbing.
作者
唐利华
郭堂勋
李其利
黄穗萍
莫贱友
Tang Lihua;Guo Tangxun;Li Qili;Huang Suiping;Mo Jianyou(Institute of Plant Protection,Guangxi Academy of Agricultural Sciences/Key Lab for Biology of Crop Diseases and Insect Pests of Guangxi,Nanning Guangxi 530007,China)
出处
《中国植保导刊》
北大核心
2018年第8期81-87,共7页
China Plant Protection
基金
广西农业科学院科技发展基金(桂农科2015YT39)
南宁市科学研究与技术开发计划项目(20162323)
南宁市青秀区科学研究与技术开发计划项目(2017040)
关键词
柑橘黄龙病
田间诊断
检测技术
citrus Huanglongbing
field diagnosis
detection technique
