摘要
目的通过观察脑灵汤对Fmr1基因敲除小鼠的水迷宫实验,探讨脑灵汤对脆性X综合征的治疗作用及可能的机制。方法选用4周龄Fmr1基因敲除型纯合子小鼠84只作为研究对象,将所选动物根据所给药物的不同分为模型组、溶媒对照组和脑灵汤治疗组,其中脑灵汤治疗组分为1 ml/(kg·d)组,2 ml/(kg·d)组,4 ml/(kg·d)组,6 ml/(kg·d)组。所选小鼠在均在饲养腹腔注射药物后行水迷宫实验,评估小鼠的行为特征并比较。结果 Morris水迷宫实验中,隐匿平台搜索实验中,模型组和溶媒对照组逃避潜伏期最长,组间差异无统计学意义;脑灵汤小鼠逃避潜伏期均低于模型组和溶媒对照组,且以4 ml/(kg·d)为最低(P<0.05)。在探索实验中,模型组与溶媒对照组的探索次数最低,脑灵汤组的平均探索次数明显高于模型组和溶媒对照组,其中以4 ml/(kg·d)脑灵汤组探索次数最高,差异有统计学意义。在可见平台实验中,各组小鼠的逃避潜伏期没有明显的组间差异。结论脑灵汤能改善Fmr1基因敲除小鼠的学习记忆能力,此可能是治疗脆性X综合征的作用机制。
Objective To observe the water maze test of Fmrl gene knockout mice by Naoling decoction, and to explore the therapeu- tic effect and possible mechanism of Naoling decoction on fragile X syndrome. Methods 84 4-week-old Fmrl gene knockout homozygous mice were selected as the study objects. The seIected animals were divided into model group, solvent control group and Naoling decoction treatment group according to the different drugs given, in which the Naoling decoction group was divided into 1 ml/（ kg · d） group and 2 ml /（kg · d） group. 4 ml/（kg· d） group （6 ml / （kg · d） group. The selected mice were fed with intraperitoneal injection of drugs. Re- suits In the Morris water maze experiment, the escape latency of the model group and the solvent control group was the longest. There was no significant difference between groups. The escape latency of Naolingtang mice was lower than that of model group and solvent control group, and the lowest value was 4 ml / （kg · d） （P 〈 0. 05）. The number of exploration experiments in the model group and the solvent con- trol group was the lowest, and the average number of exploration in the Naoling decoction group was significantly higher than that in the mod- el group and in solvent control group, with the highest number of explorations in the 4 ml/（kg · d） Naoling decoction group. The difference was significant. There was no significant difference in escape latency among groups. Conclusion Naoling decoction can improve the learn- ing and memory ability of Fmrl knockout mice. This may be the mechanism of treatment of fragile X syndrome.
作者
曾广文
陈琼
冯志坚
赵亚琳
陈盛强
ZENG Guang-wen;CHEN Qiong;FENG Zhi-jian(Guangzhou Baiyun District Third People＇s Hospital,Guangzhou,510545,China)
出处
《黑龙江医学》
2018年第8期761-762,共2页
Heilongjiang Medical Journal
基金
广州市白云区科技项目(2016-KZ-028)
关键词
脑灵汤
脆性X综合征
作用机制
Naoling decoction
Fragile X syndrome
Mechanism
