摘要
目的应用血栓弹力图仪评价不同剂量乌司他丁的体外抗凝作用。方法招募健康志愿者20名,男10名,女10名,从肘静脉采集静脉血4 mL,按乌司他丁剂量设置空白对照组、低剂量组(1000 IU/mL)、中剂量组(2000 IU/mL)和高剂量组(4000 mL/IU),同时检测血栓弹力图,并对R值、K值、α角、最大振幅(MA)和综合凝血指数(CI)参数进行统计学分析。结果与对照组比较,低、中、高剂量组的R值和K值均显著延长(P<0. 05),α角、MA和CI均显著降低(P<0. 05)。与低剂量组比较,中剂量组和高剂量组的R值均显著延长(P<0. 05),MA和CI均显著降低(P<0. 05)。针对健康男性,对照组与低剂量组在各项指标上均差异无统计学意义(P>0. 05);与对照组与低剂量组比较,中剂量组和高剂量组的R值和K值均显著延长(P<0. 05),MA和CI均显著降低(P<0. 05)。针对健康女性,与对照组比较,低、中、高剂量的R值和K值均显著延长(P<0. 05),α角、MA和CI均显著降低(P<0. 05),但低、中、高剂量组间的R值、K值、α角、MA和CI差异无统计学意义(P>0. 05)。结论乌司他丁在体外能够明显抑制凝血因子活性、降低纤维蛋白原及血小板功能,促使机体形成低凝状态。这种体外抗凝作用与剂量和性别显著相关。
Objective To evaluate the anticoagulant effect of different doses of ulinastatin （UTI） in vitro by using thromboelastography （TEG）. Methods Twenty healthy volunteers were enrolled, female and male in half. The blood samples 4mL were drew from the elbow vein and analyzed simultaneously by TEG. The control group （CG）, low-dose group （1000 IU/mL, LUG）, middle-dose group （2000 IU/mL, MUG） and high dose group （4000 IU/mL, HUG） were set up according to the dose of UTI. The value of R, K, α angle, MA and CI were analyzed statistically. Results Compared with CG, the R and K values of LUG,MUG and HUG were significantly prolonged（ P 〈0.05）, α, MA and CI were significantly decreased （ P 〈0.05）. Compared with LUG, the R values of MUG and HUG were significantly prolonged （ P 〈0.05）, MA and CI values were significantly decreased （ P 〈0.05）. For male group, there was no significant difference between mCG and mLUG in TEG （ P 〉0.05）. Compared with mCG and mLUG, the R and K value of mMUG and mHUG were significantly prolonged （ P 〈0.05）, α, MA and CI significantly were decreased （ P 〈0.05）. For female group, compared with fCG, the R and K value of fLUG, fMUG and fHUG were significantly prolonged （ P 〈0.05）, α, MA and CI were significantly decreased （ P 〈0.05）, but there was no statistical difference in R value, K value,α angle, MA and CI between the fMUG, fLUG and the fHUG （ P 〉0.05）. Conclusion Ulinastatin can significantly inhibit the activity of coagulation factor, reduce the function of fibrinogen and platelet, and thus convey anticoagulant effect in vitro , which is associated with dose and gender.
作者
宋景春
胡炜
杨洋
林青伟
曾庆波
邓星平
SONG Jing-chun;HU Wei;YANG Yang;LIN Qing-wei;ZENG Qing-bo;DENG Xing-ping(Intensive Care Unit,the 94th Hospital of PLA,Nanchang 330002,Jiangxi,China)
出处
《东南国防医药》
2018年第5期480-484,共5页
Military Medical Journal of Southeast China
关键词
乌司他丁
血栓弹力图
凝血
体外
性别
ulinastatin；thromboelastography；coagulation； vitro
gender
