摘要
目的:研究人参皂甙Rg1对传代培养雪旺细胞(SCs)增殖的影响。方法:取1~3 d SD大鼠的坐骨神经,分离SCs,于体外进行原代培养,将原代培养的SCs分别接种至含有25μg/m L人参皂甙Rg1的培养液(观察组)和含有生理盐水的培养液(对照组)中,进行传代培养,对传代培养的SCs进行细胞计数分析。结果:传代SCs计数表明,含25μg/m L人参皂甙Rg1的观察组SCs细胞数多于对照组(t=9.831,P=0.000)。结论:人参皂甙Rg1促进传代培养SCs增殖,有助于周围神经的再生修复。
Objective：To study how ginsenoside Rgl affects the proliferation of Schwann cells （SCs） by subcultural solution. Methods：The SCs were isolated from the sciatic nerves of SD rats which have lived for 1 - 3 days and subcultured in vitro ; the subcultured SCs were inoculated into the 25μg/ml ginsenoside Rgl solution （the observation group） and the normal saline solution （the control group） respectively and were sub- cultured respectively; The SCs were counted and analyzed. Results：The Result showed that there were more SCs in the observation group than in the control group（ t=9. 831, P -0. 000）. Conclusion： Ginsenoside Rgl will promote the proliferation of subcultured SCs and is beneficial to the regeneration and repair of peripheral nerves.
作者
胡华麟
HU Hualin(Anqing Medical and Pharmaceutical College,Anqing 246052,China)
出处
《包头医学院学报》
CAS
2018年第8期86-86,89,共2页
Journal of Baotou Medical College
基金
2016年安庆医药高等专科学校校级自然科学研究重点项目(2016xzz01)
