摘要
利用直接浸提法和凯氏定氮蒸馏装置,建立湿米粉中双乙酸钠的高效液相色谱法快速检测。湿米粉中的双乙酸钠经过磷酸酸化后转化为酸,通过凯氏定氮蒸馏装置、超声浸提法,用磷酸溶液(1 mol/L)调p H为3.0左右,采用高效液相色谱法进行测定。结果表明,直接浸提法双乙酸钠的回收率为98.32%~103.13%,相对标准偏差为0.322%~0.877%;凯氏定氮蒸馏法双乙酸钠的回收率为98.12%~102.52%,相对标准偏差为0.317%~1.750%,说明凯氏定氮蒸馏法简便快捷、稳定可靠,适用于鲜湿米粉中双乙酸钠含量的测定。
Using Direct extraction and kjeldahl distillation apparatus, a method for the simultaneous determination of sodium diacetate for wet rice noodles by high performance liquid chromatography（HPLC） was established. Methods the sodium diacetate in the wet rice noodles was converted to the corresponding acids after phosphoric acid acidification,After that,acid was collected in the Kjeldahl nitrogen distillation unit. The p H of the distillates was adjusted to around 3.0 by phosphoric acid solution（1 mol/L）. Finally, HPLC was used for the determination. Recoveries of direct extraction were between 98.32% ~103.13%,and the RSD was in the range of 0.322%~0.877%; Recoveries of kjeldahl nitrogen distillation were between 98.12%~102.52%,and the RSD was in the range of 0.317%~1.750%（n=10）. Conclusion The method of kjeldahl distillation is simple,fast, stable and reliable, and it is suitable for the determination of sodium diacetate content in wet rice noodles.
作者
郑娟梅
莫紫梅
刘珈伶
杨黎
赵就彬
ZHENG Juan-mei;MO Zi-mei;LIU Jia-ling;YANG Li;ZHAO Jiu-bin(Guangxi-ASEAN Center for Food and Drug Safety Control,Nanning 530021,China)
出处
《湖北农业科学》
2018年第17期93-95,125,共4页
Hubei Agricultural Sciences
基金
广西食品药品监督管理局科学研究项目(桂食药科2018-7)
广西地方标准制定(修订)项目(桂地标食2017006号)
广西地方标准制定(修订)项目(桂地标食2017007号)
关键词
直接浸提法
凯氏定氮蒸馏法
双乙酸钠
湿米粉
高效液相色谱
direct extraction
kjeldahl distillation apparatus
wet rice noodles
high performance liquid chromatography（HPLC）
