摘要
为明确落羽杉属树木赤枯病的致病菌种类,对采自南京中山植物园及周边地区的病叶进行病原菌分离,结合形态学特征与分子生物学技术对其进行鉴定,并分析病原菌的生物学特性。结果表明,共获得9种菌种,分别标号为1~9,其中2号菌株检出率最高,占总数的36.40%,用该菌株侵染健康叶片后发病症状与自然发病症状一致,并可再次分离到2号菌株,符合柯赫氏法则;形态学鉴定结果显示,病原菌分生孢子为梭形、直或弯曲,5个细胞,顶胞和尾胞均为圆锥形,无色透明;r DNA-ITS序列分析结果表明,该菌株与斑污拟盘多毛孢Pestalotiopsis maculans同源性最高,为99.00%。结合形态特征与分子鉴定最终确定落羽杉赤枯病病原菌为斑污拟盘多毛孢。生物学特性分析结果表明,该病原菌在25℃、p H 6~7的PDA培养基上长势最好;在以淀粉为碳源、谷氨酸为氮源的培养基上生长最快。
In order to identify the pathogen causing red blight in Taxodium, the pathogen was isolated from the diseased leaves collected from Nanjing Botanical Garden, Memorial Sun Yat-Sen and its surrounding areas, and identified with morphological and molecular biological techniques. The biological characteristics of the pathogen were also determined. The results showed that nine strains were obtained, and the frequency of No. 2 fungus（36.40%） was the highest among all isolates. The symptoms of Taxodium inoculated with No. 2 fungus were similar to that observed under natural conditions, and No. 2 fungus could be isolated from these leaves in line with Koch＇s rule. The conidia were fusiform,straight or curved. Each conidium contained five cells, with the apical cells hyaline and colorless. In addition, the r DNA-ITS sequence of the pathogen showed 99.00% homology with that of Pestalotiopsis.Therefore, based on the morphological and molecular analyses, the pathogen causing red blight in Taxodium was defined as Pestalotiopsis maculans. Furthermore, the growth of P. maculans was best at 25℃,p H 6-7 on PDA medium, and starch glutamic acid were most suitable carbon source and nitrogen source.
作者
张凡
冯婷
韩正敏
殷云龙
华建峰
於朝广
Zhang Fan;Feng Ting;Han Zhengmin;Yin Yunlongl;Hua Jianfeng;Yu Chaoguang(Institute of Botany,Jiangsu Province and Chinese Academy of Sciences,Nanjing 210014,Jiangsu Province,China;College of Forestry,Nanjing Forestry University,Nanjing 210037,Jiangsu Province,China)
出处
《植物保护学报》
CAS
CSCD
北大核心
2018年第4期900-907,共8页
Journal of Plant Protection
基金
江苏省农业科技自主创新资金项目(CX(16)1005)
江苏省创新能力建设计划(科技设施类)(BM2015019)
国家自然科学基金(31570593)
关键词
落羽杉
赤枯病
拟盘多毛孢
分离鉴定
生物学特性
Taxodium
red blight
Pestalotiopsis sp.
isolation and identification
biological characteristics
