摘要
目的 分析泛素特异性蛋白酶18（USP18）在人胃癌细胞株中的表达及其作用,探讨其与胃癌发生、发展的关系.方法 蛋白质印迹法和实时荧光定量聚合酶链反应（RT-PCR）检测永生化胃黏膜上皮细胞株GSE和胃癌细胞株（AGS、MKN45、MKN25、BGC823、BGC803、SGC7901）中USP18蛋白和mRNA表达水平;CCK-8法和Transwell实验检测USP18在胃癌细胞侵袭和增殖中的作用.结果 GSE细胞中USP18 mRNA的含量表达低于各胃癌细胞株（F=794.052,P＜0.000 1）.6种胃癌细胞株中,BGC823和BGC803中USP18 mRNA的相对含量高,分别为17.62±0.55和13.52±0.50,而在MKN28和MKN45细胞中的含量低,分别为1.40±0.17和4.23±0.26.在蛋白水平上,USP18在GSE细胞中的表达最低,在6株胃癌细胞中,USP18在侵袭能力较强的SGC7901和BGC803细胞中的表达最高,而在AGS和MKN45细胞中的表达最低.与对照组相比,干扰USP18后SGC7901和BGC803的侵袭和增殖能力降低,差异均具有统计学意义（P＜0.01）.结论 USP18在侵袭能力较强的胃癌细胞株中高表达,下调USP18能抑制胃癌细胞的侵袭和增殖.
Objective To analyze the expression and role of ubiquitin specific peptidase 18 （USP18） in gastric cancer cells,and to investigate the relationship between the development of gastric cancer and USP18.Methods The levels of USP18 protein and mRNA expression in immortalized gastric mucosa epithelial GSE cell lines and gastric cancer cell lines （AGS,MKN45,MKN25,BGC823,BGC803,SGC7901） were detected by using reverse transcription-polymerase chain reaction （RT-PCR） and Western blot,respectively.The role of USP18 in the invasion and proliferation of gastric cancer cells was analyzed by using CCK8 and Transwell assays.Results The mRNA level of USP18 was lower in GSE cell lines than that in gastric cancer cells （F =794.052,P 〈 0.000 1）.In six gastric cancer cell lines,mRNA level of USP18 was relatively high in BGC823 （17.62±0.55） and BGC803 （13.52±0.50） cell lines,and low in MKN28 （1.40±0.17） and MKN45 cell lines （4.23±0.26）.As for the protein level,the expression of USP18 was lowest in GSE cell line.In six gastric cancer cell lines,the expression of USP18 was the highest in more aggressive SGC7901 and BGC803 cell lines and the lowest in AGS and MKN45 cells.Compared with the control group,interference of USP18 decreased the invasion and proliferation abilities of SGC7901 and BGC803 cell lines （P 〈 0.01）.Conclusion USP18 is highly expressed in more invasive gastric cancer cells,and the downregulation of USP18 can suppress the invasion and proliferation of gastric cancer cells.
作者
钟显飞
易礼智
邓学杰
程征宇
王琴
王舰梅
Zhong Xianfei;Yi Lizhi;Deng Xuejie;Cheng Zhengyu;Wang Qin;Wang Jianmei(Department of Gastroenterology,the People＇ s Hospital of Leshan City,Leshan 614000,China（Zhong XF,Yi LZ,Deng X J,Cheng ZY,Wang Q;Pathological Teaching and Research Office,Southwest Medical University,Luzhou 646000,China（Wang JM)
出处
《肿瘤研究与临床》
CAS
2018年第7期433-437,共5页
Cancer Research and Clinic
基金
四川省卫生和计划生育委员会科研课题（150070）
泸州市人民政府-西南医科大学科技战略合作项目（2016LZXNYDJ29）
关键词
胃肿瘤
泛素特异性蛋白酶18
肿瘤侵润
细胞增殖
Stomach neoplasms
Ubiquitin specific peptidase 18
Neoplasm invasiveness
Cellproliferation
