摘要
目的：通过UPLC-MS检测小鼠主要脏器中石斛碱的含量,探讨石斛碱在小鼠体内的分布特征。方法：建立组织样品中石斛碱的分析方法,小鼠灌胃给予石斛碱（剂量60 mg·kg-1）,分别于0,30,60,120 min收集小鼠心、肝、脾、肺、肾、脑和肠组织,样品处理后采用Q-Exactive型四极杆-静电场轨道阱高分辨质谱系统分析,Hypersil Gold C18色谱柱（2.1 mm×150 mm,1.9μm）,流动相0.1%甲酸水溶液-乙腈梯度洗脱,流速0.3 m L·min-1,可加热电喷雾离子源（HESI）正离子模式检测石斛碱含量,选取盐酸伪麻黄碱为内标。结果：小鼠各组织样品中内源性物质对石斛碱含量测定无干扰,方法学考察符合要求。石斛碱在小鼠心、肝、脾、肺、肾、脑和肠组织中均有分布,30 min均已达峰值,此时肝脏样本中石斛碱质量浓度最高（1 241.69±78.64）μg·L-1。60 min时各组织样本中石斛碱含量已显著降低,此时空肠组织样本中石斛碱质量浓度最高（166.15±10.80）μg·L-1。120 min时各组织样本中石斛碱含量进一步降低,此时以回肠组织样本中石斛碱质量浓度最高（118.25±8.51）μg·L-1。结论：石斛碱在小鼠体内的组织分布较为广泛,其中肠组织和肝脏中药物浓度较高。在脑组织中的分布情况提示石斛碱能够透过血脑屏障,为阐述其具有神经药理作用提供了实验依据。
Objective： To establish UPLC-MS analysis method for the determination of dendrobine in the main organs of C57 BL/6 J mice,and explore the tissue distribution characteristics of dendrobine in vivo. Method：At 0, 30, 60, 120 min after gavage, the main organs（ heart, liver, spleen, lung, kidney, brain and intestine） were collected and precipitated by acetonitrile,and the supernatant was detected with UPLC-MS. The chromatography separation was performed on a Hypersil Gold C18 column（ 2. 1 mm × 150 mm,1. 9 μm） with acetonitrile-0. 1% formic acid in water for gradient elution. Heated electrospray ionization source（ HESI） was applied and operated in the positive ion mode. Meanwhile, pseudoephedrine hydrochloride was used as the internal standard for determining. Result： The endogenous substances in the tissues of mice had no interference for the assay of dendrobine,and the methodological study met the requirements. Dendrobine could distribute in heart,liver,spleen,lung,kidney,brain and intestine tissue of mice,and its content reached the peak of content at30 min after gavage,and then decreased significantly with time. The distributions of dendrobine at different time points after administration were distinct. Dendrobine was mainly distributed in liver tissue at 30 min（ 1 241. 69 ±78. 64） μg·L-1,and in jejunum and ileum at 60,120 min（ 166. 15 ± 10. 80） μg·L-1 and（ 118. 25 ±8. 51） μg·L-1,respectively. Conclusion： Dendrobine is widely distributed in mice,and its concentration in intestinal tissue and liver is high. The distribution in brain suggests that dendrobine can penetrate the blood-brain barrier,and this study can provide a basis for elucidating its neuropharmacological action.
作者
鲁艳柳
刘浩
曾瑶
黄思
王建秋
徐亚沙
何芋岐
陆远富
LU Yan-liu;LIU Hao;ZENG Yao;HUANG Si;WANG Jian-qiu;XU Ya-sha;HE Yu-qi;LU Yuan-fu(Key Laboratory of Basic Pharmacology,and Joint International Research Laboratory of Ethnomedicine,Ministry of Education,Zunyi Medical University,Zunyi 563003,China)
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2018年第18期134-139,共6页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(81660685)
贵州省科技重大专项(黔科合重大专项字[2015]6010)
贵州省科学技术基金项目(黔科合JZ字[2015]2010号)
贵州省教育厅自然科学研究项目(黔教合KY字[2015]373号)
遵义医学院优秀青年人才项目(15zy-004)
关键词
金钗石斛
石斛碱
组织分布
盐酸伪麻黄碱
方法学考察
肝脏
超高效液相色谱-串联质谱法
Dendrobium nobile
dendrobine
tissue distribution
pseudoephedrine hydrochloride
methodological investigation
live
ultra-performance liquid chromatography-tandem mass spectrometry
