摘要
目的探究芝芪菌质及其各部位免疫活性。方法芝芪菌质为灵芝菌丝-黄芪药渣的固体发酵复合体。通过小鼠碳粒廓清实验,鸡红细胞吞噬功能实验,小鼠血清溶血素、免疫球蛋白M(IgM)和免疫球蛋白G(IgG)的含量检测,以及小鼠脾淋巴细胞增殖实验,筛选了芝芪菌质水提液,芝芪菌质水浸液,芝芪菌质水提液经大孔树脂分离的部位(水洗脱液、40%乙醇洗脱液和95%乙醇洗脱液)的非特异性和特异性免疫活性。结果碳粒廓清实验中,K值最高为水浸液和水提液组的0.23,α值最高为水提液组的2.44。吞噬指数最高为水浸液和水提液组的0.36,鸡红细胞吞噬百分率最佳为水浸液组的47.25%。小鼠溶血素含量最高为水洗脱组的0.4。IgM含量最高为水提液组的34.19 mg·L^(-1),而IgG最高则是水浸液组的44.50 mg·L^(-1)。淋巴细胞增殖指数最高为40%乙醇洗脱液组的1.23。结论芝芪菌质水提液、芝芪菌质水浸液和40%乙醇洗脱液具有显著的免疫增强功效,为芝芪菌质制成增强人体抵抗力的功能性食品及药品提供实验数据和理论参考。
Objective To investigate immunocompetence of zhiqi fungal substance and its parts. Methods Zhiqi fungal substance was the solid fermentation compound from Ganoderma Lucidum myceliumand Radix Astragali residue. The mouse carbon clearance,phagocytosis of chicken red blood cells,determination of hemolysin,IgM and IgG in mice serum,and mouse spleen lymphocyte proliferation were used to screen water extraCT,aqueous infusion and separated parts of zhiqi fungal substance. Nonspecific and specifc immunity aCTivities of water extraCT of zhiqi fungal substance were separate by macroporous resin. The separated parts were made up of water eluent,40%ethanol eluent and 95% ethanol eluent with macroporous resinseparating water extraCT of zhiqi fungal substance. Results According to the experiment of carbon clearance,it was showed that the highest K value is 0. 23 in aqueous infusion and water extraCT group,the highest alpha value is 2. 44 of water extraCT group,the highest phagocytic index is 0. 36 in aqueous infusion and water extraCT group,and the best phagocytic percentage of chicken red blood cells is 47. 25% of water extraCT group. In addition,the highest content of hemolysin in mice was 0. 4 of water eluent group; the highest content of IgM was 34. 19 mg·L^（-1） from water extraCT group,while the highest IgG was 44. 50 mg·L^（-1） in aqueous infusion group; the highest lymphocyte proliferation index was 1. 23 in the 40% ethanol eluent group.Conclusions The water extraCT,aqueous infusion and 40% ethanol eluent from zhiqi fungal substances have remarkable immunity enhancement effeCT,and provide experimental data and theoretical reference for making zhiqi fungal substance into funCTional food and drug with body resistance strengthened.
作者
阮鸣
喻斌
霍光明
张李阳
刘维周
RUAN Ming;YU Bin;HUO Guangming;ZHANG Liyang;LIU Weizhou(Jiangsu Provincial Key Construction Laboratory of Special Biomass Waste Resource Utilization,School of Food Science,Nanfing Xiaozhuang University,Nanfing,Jiangsu 211171,China;Jiangsu Key Laboratory for Pharmacology and Safety Evaluation of Chinese Materia Medica,Nanfing University of Chinese Medicine,Nanfing,Jiangsu 210023,China)
出处
《安徽医药》
CAS
2018年第10期1869-1873,共5页
Anhui Medical and Pharmaceutical Journal
基金
国家自然科学基金面上项目(81573713)
江苏省自然科学基金项目(BK20150088
BK20151564)
江苏省高校重点建设实验室(苏教高[2016]8)
关键词
灵芝
中草药
黄芪
发酵
免疫调节
吞噬作用
小鼠
大鼠
Ganoswema lucidum
Drugs
chinese herbal
Astragalus membranaceus
hnmunomodulation
Fermentation
Phagocytosis
Mice
Rats
