摘要
目的:研究加味小柴胡汤各提取部位的有效成分对幽门螺杆菌(Hp)的体外抑制效果。方法:对6-姜酚、盐酸小檗碱、黄芩苷、柴胡皂苷a等有效成分进行抑菌试验,琼脂扩散实验采用牛津杯的方法,按一定菌浓度混合培养基铺菌层,每孔100μl药液,培养3 d,测量抑菌圈直径;液体稀释实验采用梯度稀释法,在试管内加入1 ml含药培养基和50μl菌液,培养3d,观察浑浊情况并计算最小抑菌浓度(MIC)。结果:6-姜酚、盐酸小檗碱、黄芩苷、柴胡皂苷a等有效成分抑菌效果均优于阳性对照组(P<0.01);盐酸小檗碱组的抑菌效果优于其他3个给药组(P<0.05)。结论:确定了6-姜酚、盐酸小檗碱、黄芩苷、柴胡皂苷a等有效成分均对Hp有抑制作用,为研究加味小柴胡汤方抑制Hp的物质基础提供科学依据。
Objective： To investigate the inhibitory effect of the effective components in the different extracts of modified Xiao Chaihu decoction on Helicobacter pylori （ H. pylori） in vitro. Methods： 6-Gingerol, berberine hydroehloride, baiealin, and saikosapo- nin a were tested for bacteriestasis. Agar diffusion test was carried out by using Oxford cup method. The medium was mixed with a cer- tain concentration of bacteria with 100 μl per well and cultured for 3 days, and then the diameter of inhibition zone was measured. The liquid dilution experiment was performed with gradient dilution ,50 μl bacteria liquid and 1 ml medium containing drug were mixed and cultured for 3 days, and the turbidity was observed and the minimal inhibitory eoncentratio （MIC） was calculated. Results ： The anti- bacterial effects of 6-gingerol, berberine hydroehloride, baicalin and saikosaponin a were all better than that of the positive control group （ P 〈 0.01 ）, and berberine hydroehloridc group had better antibacterial effect than the other three groups （ P 〈 0.05 ）. Conclusion： The results proved that 6-gingerol, berberine hydrochloride, baicalin and saikosaponin a all have inhibitory effects on H. pylori, which provide scientific evidence for the study on the material basis of modified Xiao Chaihu decoction used for inhibiting H. pylori.
出处
《中国药师》
CAS
2018年第9期1559-1562,共4页
China Pharmacist
关键词
加味小柴胡汤
幽门螺杆菌
抑制作用
6-姜酚
盐酸小檗碱
黄芩苷
柴胡皂苷A
Modified Xiao Chaihu decoction
Helicobacterpylori
Inhibition
6-Gingerol
Berberine hydrochloride
Baicalin
Sai- kosaponin a
