摘要
目的:探讨心痛泰对急性心肌缺血大鼠新生微血管密度及血管内皮活性物质的影响。方法:将SD大鼠随机分成假手术组,模型组,心痛泰低、中、高剂量组,麝香保心丸组。除假手术组外,其余组大鼠均予以结扎冠状动脉左前降支制备成急性心肌缺血损伤模型,成模后分别给予蒸馏水,心痛泰低、中、高剂量药液及麝香保心丸进行灌胃治疗2周。HE染色光镜观察心肌形态结构改变;ELISA试剂盒检测血清中一氧化氮(NO)、一氧化氮合酶(NOS)、内皮素1(ET-1)含量;免疫组化法测定新生微血管密度。结果:与假手术组相比,模型组血清中NO、NOS表达减少(P<0.01),ET-1表达增加(P<0.01),缺血心肌微血管密度表达增加(P<0.05);与模型组相比,各用药组均可升高血清中NO、NOS含量(P<0.01),降低ET-1含量(P<0.01),促进缺血区微血管密度的表达(P<0.05)。结论:心痛泰具有保护缺血心肌之效,这可能与其提高血清中NO、NOS含量、减少ET-1的含量、促进缺血区新生血管生成相关。
Objective： To investigate the effect of Xintongtai on microvascular density and vascular endothelial active substances in rats with acute myocardial ischemia. Methods： Sprague-Dawley rats were randomly divided into sham-operation group,model group,low-,middle-,and high-dose Xintongtai groups,and Shexiang Baoxin pill group. All rats except those in the sham-operation group were treated with ligation of the left anterior descending coronary artery to establish a model of acute myocardial ischemia and were then given distilled water,low-,middle-,and high-dose Xintongtai liquid,or Shexiang Baoxin pills by gavage for 2 weeks. HE staining and a light microscope were used to observe the morphological and structural changes of the myocardium; ELISA was used to measure the serum levels of nitric oxide（ NO）,nitric oxide synthase（ NOS）,and endothelin-1（ ET-1）; immunohistochemistry was used to measure microvascular density. Results： Compared with the sham-operation group,the model group had significant reductions in the serum levels of NO and NOS（ P 〈0. 01） and a significant increase in the serum level of ET-1（ P〈 0. 01）,as well as a significant increase in microvascular density in the ischemic myocardium（ P 〈0. 05）. Compared with the model group,each treatment group had significant increases in the serum levels of NO and NOS（ P 〈0. 01）,a significant reduction in the serum level of ET-1（ P 〈0. 01）,and a significant increase in microvascular density in the ischemic region（ P 〈0. 05）. Conclusion： Xintongtai exerts a protective effect on the ischemic myocardium,possibly by increasing the serum levels of NO and NOS,reducing the serum level of ET-1,and promoting neovascularization in the ischemic region.
作者
吴钟琴
李雅
郭志华
任欣
邓育兵
宋汝汝
WU Zhong - qin1 , LI Ya2, GUO Zhi - hua2, REN Xin2, DENG Yu - bing3 , SONG Ru - ru4(1.Hengyang Hospital of Traditional Chinese Medicine, Hengyang 421000, Hunan, China ; 2. Hunan University of Chinese Medicine, Changsha 410208 ,Hunan, China; 3. Hunan Provincial Hospital of Traditional Chinese Medicine ,Zhuzhou 412000 ,Hunan ,China; 4. Huixian Hospital of Traditional Chinese Medicine, Huixian 453600, Henan, Chin)
出处
《湖南中医杂志》
2018年第8期174-178,共5页
Hunan Journal of Traditional Chinese Medicine
基金
湖南省教育厅科学研究项目(编号:16A158)
关键词
急性心肌缺血
SD大鼠
心痛泰
新生微血管密度
实验研究
acute myocardial ischemia
Sprague- Dawley rat
Xintongtai
microvascular density
experimental study
