摘要
目的探讨冠心病患者白细胞分化抗原(CD14)基因多态性与超敏C反应蛋白水平(Hs-CRP)的相关性。方法选取2016年5月—2017年5月该院收治的冠心病患者120例,应用合酶链反应-限制性片段长度多态性(PCR-RFLP)技术对患者CD14基因进行测定,同时检测患者体内Hs-CRP水平,并分析CD14基因多态性与超敏C反应蛋白水平间的关系。结果分析CD14基因的酶切图像结果以及基因型,结果显示:CD14基因(-260)多态性PCR扩增片段的大小为560 bp。对120例患者的基因型进行统计,主要基因型为TT、TC、CC,患者例数依次为53例(44.2%)、41例(34.1%)、26例(21.7%),基因型符合Hardy-Weinberg平衡;CD14基因(-260)CT的多态性与年龄、性别、吸烟史、高血压病史、糖尿病病史、LDL-C、HDL-C、TC、TG间差异无统计学意义(P>0.05);TT、TC、CC各基因型患者的Hs-CRP的血浆水平分别为(4.5±2.0)、(2.1±1.0)、(2.9±1.1)mg/L,3组患者比较差异有统计学意义(P<0.05)。结论 TT纯合子的冠心病患者炎症反应最强烈,因此CD14基因变异与超敏C蛋白水平密切相关。
Objective To study the correlation between the CD14 gene polymorphism and hypersensitivity C reactive protein level of patients with coronary heart disease. Methods 120 cases of patients with coronary heart disease admitted and treated in our hospital from May 2016 to May 2017 were selected, and the CD14 gene polymorphism was measured by PCR-RFLP, at the same time, the Hs-CRP level of patients was tested, and the correlation between the CD14 gene polymorphism and hypersensitivity C reactive protein level was analyzed. Results The analysis results of enzyme cut image of CD14 gene and gene type showed that the size of（-260） polymorphic PCR amplification fragment of CD14 gene was 560 bp, and the statistics of genes of 120 cases of patients showed that the major gene types were TT,TC, CC, and the case numbers were 53 cases（44.2%）, 41 cases（34.1%）, 26 cases（21.7%）, and the gene type was in line with Hardy-Weinberg balance, and the polymorphism of CD14 gene（-260） CT had no statistical significance with the age, gender, smoking history, hypertension history, diabetes history, LDL-C, HDL-C, TC, TG（P〉0.05）, and blood plasma levels of Hs-CRP of patients with TT, TC, CC were respectively（4.5±2.0）,（2.1±1.0）,（2.9±1.1） mg/L, and the differences between the three groups were statistically significant（P〈0.05）. Conclusion The inflammatory reactions of coronary heart disease patients with TT homozygote is the strongest, therefore, the CD14 gene mutation is closely related to the hypersensitivity C protein level.
作者
邹艳惠
刘颖
李默郁
陈立涛
张雪莲
ZOU Yan -hui;LIU Ying;LI Mo-yu;CHEN Li-tao;ZHANG Xue-lian(Department of Cardiology,Jilin Provincial People＇s Hospital,Changchun,Jilin Province,130021 China)
出处
《系统医学》
2018年第13期7-8,21,共3页
Systems Medicine
基金
吉林省卫生厅课题
项目名称:冠心病与CD14相关性及他汀类药物干预作用的研究(2010ZC038)
