摘要
目的:优化微波提取黄精药材中总皂苷的工艺,为黄精药材质量评价提供参考依据。方法:以紫外分光光度法为检测手段,采用单因素实验法与Box-Benhken响应面法相结合,研究了料液比、提取时间、乙醇浓度和微波加热功率等工艺参数对黄精总皂苷得率的影响。通过全波长扫描比较了人参皂苷Rb1、薯蓣皂苷元和菝葜皂苷元三种对照品与样品最大吸收波长。结果:响应面优化后得到的最佳提取条件为:乙醇浓度为50%,料液比为1∶16,提取时间为40 s。扫描结果显示菝葜皂苷元和样品的最大吸收波长均为460 nm。结论:采用响应面法优化黄精总皂苷的提取方法切实可行,为黄精的提取条件提供理论依据。
Objective： The process of extracting total saponins from Rhizoma Polygonati was optimized to provide reference for the quality evaluation. Methods： Based on the single-factor tests,extraction conditions of total saponins were optimized by Box-Behnken design,and the anti-inflammatory activity was assessed by in vitro inflammatory model of mouse macrophages. Ultraviolet spectrophotometry was used to determine the total saponins. The maximum absorption wavelength of ginsenoside Rb1,diosgenin and sarasin was compared by the full wavelength scanning. Results： The optimum extraction condition after the response surface optimization was： ethanol concentration was 50%,the mixture ratio was 1： 16,and the extraction time was 40 s. The results showed that the maximum absorption wavelength was 460 nm,and the maximum absorption wavelength was 460 nm. Conclusion： The response surface method was used to optimize the extraction of total saponins,and it was feasible to provide the theoretical basis for the extraction conditions of Rhizoma Polygonati.
作者
赵丽蓉
罗汉
相英龙
孙慧娟
梅桂林
方成武
ZHAO Li-rong;LUO Han;XIANG Ying-long;SUN Hui-juan;MEI Gui-lin;FANG Cheng-wu(College of Pharmacy,Anhui University of Chinese Medicine,Hefei 230012,China;Bozhou Institute of Traditional Chinese Medicine,Anhui Academy of Chinese Medicine,Bozhou 236800,China)
出处
《中国现代中药》
CAS
2018年第8期1010-1015,共6页
Modern Chinese Medicine
基金
中医药行业科研专项(201207002)
关键词
黄精
总皂苷
微波提取
响应面
Rhizoma Polygonati
total saponins
microwave extraction
response surface
