摘要
【目的】优化乙醇/(NH_4)_2SO_4双水相体系提取藏药瑞香狼毒多糖的工艺参数,并分析瑞香狼毒多糖的抑菌及抗氧化活性,为瑞香狼毒功能产品的研发及多元化应用提供理论依据。【方法】以不同的料(g)液(mL)比(1∶30,1∶40,1∶50)、提取温度(45,55,65℃)和提取时间(20,30,40min)作为考察因素,以多糖的提取率作为评价指标,采用响应曲面优化分析确定瑞香狼毒多糖最佳提取工艺参数。利用硫酸亚铁-水杨酸氧化法和邻苯三酚自氧化法分别评估瑞香狼毒多糖(质量浓度分别为0.6,1.2,1.8,2.4,3.0,3.6mg/mL)对羟基自由基(·OH)和过氧负离子自由基(·O_2^(-2))的体外清除能力。采用滤纸片扩散法研究瑞香狼毒多糖(质量浓度为0.3和3.0mg/mL)的抑菌活性。【结果】料(g)液(mL)比1∶40、提取温度55℃、提取时间30min为最优的工艺参数,在该条件下瑞香狼毒多糖的提取率最高,达15.34%。3.0mg/mL瑞香狼毒多糖对·OH的清除率最大,为29.4%;2.4mg/mL瑞香狼毒多糖对·O_2^(-2)的清除率最大,为24.7%。抑菌试验结果表明,3.0mg/mL瑞香狼毒多糖对金黄色葡萄球菌和大肠杆菌高度敏感,对枯草芽孢杆菌中度敏感。【结论】确定了瑞香狼毒多糖乙醇/(NH4)2SO4双水相体系提取的最佳工艺参数,建立了因素与提取率之间的二次函数模型;瑞香狼毒多糖具有一定的体外抑菌、抗氧化活性。
[Objective] In this work, the extraction parameters of Stellera chamaejasrne polysaccharides （SCP） using the aqueous two phase system of ethanol/ammonium sulphate were optimized and the antibac- terial and antioxidant activities of SCP were evaluated to provide theoretical basis for research,development and diversification of the products. [Method] The optimal extraction parameters were determined using re- sponse surface method for extraction temperature （45,55 and 65℃） ,extraction time （20,30 and 40 min） and ratio of liquid to materials（1 ＂ 30,1 ＂ 40 and 1 .＂ 50） with SCP yield as index. The scavenging effect of hydroxyl radical and superoxide radical were detected with the oxidation of ferrous sulfate-salicylic acid and self-oxidation of pyrogallic acid system under the mass concentrations of 0.6,1.2, 1.8,2.4,3.0 and 3.6 mg/mL. The antibacterial activities at mass concentrations of 0.3 and 3.0 mg/mL were determined by ill- ter paper diffusion method. [Result] The optimum extraction conditions were extraction temperature 55 ℃ ,extraction time 30 min and ratio of liquid to materials 1 ： 40 （g ： mL） with the highest SCP yield of15.34 %. SCP exhibited significant protection against hydroxyl radical and superoxide ion induced oxidative damage and the maximum scavenging activities were 29.4% （3.0 mg/mL） and 24.7% （2.4 mg/mL）, re- spectively. The 3.0 mg/mL SCP presented high sensitivity to Colibacillus and Staphylococcus aureus, but medium sensitivity to Bacillus subtilis. [Conclusion] The optimum SCP extraction parameters by the a- queous two phase system of ethanol/ammonium sulphate were determined and a quadratic multinomial re- gression modet between extraction factors and yield was established. SCP showed antioxidant and antimi- crobial activities in vitro.
作者
祁小妮
马梅
李振亮
党金宁
董艳娇
QI Xiaoni;MA Mei;LI Zhenliang;DANG Jinning;DONG Yanjiao(Department of Chemistry and Life Sciences,Gansu Normal University for Nationalities,Gannan,Gansu 747000,China)
出处
《西北农林科技大学学报（自然科学版）》
CSCD
北大核心
2018年第8期123-130,138,共9页
Journal of Northwest A&F University(Natural Science Edition)
基金
甘肃省高等学校科研项目(2016A-093)
关键词
瑞香狼毒
多糖提取
抑菌活性
抗氧化活性
Stellera chamaejasme
polysaccharides extraction
antioxidant activity
antibacterial activity
