摘要
为了解猪流行腹泻病毒(PEDV)的遗传变异,试验对来自安徽省不同地区8个猪场病料样品,利用RT-PCR方法,扩增PEDV ORF3基因,测序并进行序列分析。结果显示,8份样品均扩增出包含ORF3基因全长(675 bp)的目的片段,核苷酸序列同源性为98.5%~100%;它们与疫苗株(attenuated DR13、CH-BJ-2011 truncated)及CV777株和LZC株的同源性较低(95.1%~98.1%),与2011年后的中国及美国分离株存在较高同源性(98.2%~100%)。提示8个PEDV分离株均为强毒株。
To study the genetic variation of porcine epidemic diarrhea virus （PEDV） , the ORF3 genes of 8 PEDV field strains were cloned, sequenced and analyzed, which were isolated from different regions of Anhui Province from 2013 to 2014. The results showed that the ORF3 genes of 8 PEDV field strains were all 675 bp, and had 98.5%~100% nucleotide identity. The ORF3 genes of the 8 PEDV field strains had the lower homology （95.1%~98.1%） with attenuated DR13, CH-BJ-2011 truncated, CV777 and LZC strains, had the higher homology （98.2%~100%） with the reference PEDV strains isolated after 2011 from China and America. The re-sults suggested that the 8 PEDV field strains were all virulent strains.
作者
潘孝成
沈学怀
赵瑞宏
胡晓苗
戴银
周学利
侯宏艳
张丹俊
PAN Xiaocheng;SHEN Xuehuai;ZHAO Ruihong;HU Xiaomiao;DAI Yin;ZHOU Xueli;HOU Hongyan;ZHANG Danjun(Institute of Animal Husbandry and Veterinary Science,Anhui Academy of Agricultural Science,Hefei 230031,China)
出处
《养猪》
2018年第5期105-108,共4页
Swine Production
基金
安徽省重点研究与开发计划项目(1704a07020066)
安徽省农业科学院院所共建团队项目(18C0430)
安徽省生猪产业技术体系项目
安徽省科技重大专项(17030701008)
