摘要
目的探讨黄芪甲苷对慢性心力衰竭大鼠心肌细胞凋亡及磷酸化缝隙连接蛋白43(P-Cx43)表达水平的影响。方法选取SD雄性大鼠63只,采用腹主动脉缩窄法建立慢性心力衰竭模型,将造模成功大鼠随机分为模型组、曲美他嗪组、黄芪甲苷组,各21只;对照组21只接受假手术。黄芪甲苷组给予60 mg/kg黄芪甲苷,1次/d,灌胃给药;曲美他嗪组给予10 mg/kg盐酸曲美他嗪,1次/d,灌胃给药;模型组和对照组分别给予20 mg/kg蒸馏水,1次/d,灌胃;各组大鼠连续给药4周。对大鼠心脏功能、血流动力学指标、心脏重量/体质量比(HW/BW)、左心室重量/体质量比(LVW/BW)、心肌细胞凋亡指数(AI)、心肌组织P-Cx3表达水平进行检测。结果治疗后,与模型组比较,各组大鼠LVEF水平较高,且黄芪甲苷组>曲美他嗪组(P<0.05);各组大鼠IVSd、LVEDd、LVEDs水平较低,且黄芪甲苷组<曲美他嗪组,差异有统计学意义(P<0.05);各组大鼠LVSP、+dp/dtmax、-dp/dtmax水平较高,且黄芪甲苷组>曲美他嗪组(P<0.05);各组大鼠LVEDP水平较低,且黄芪甲苷组<曲美他嗪组(P<0.05);各组大鼠HW/BW、LVW/BW水平较低,且黄芪甲苷组<曲美他嗪组(P<0.05);各组大鼠心肌细胞AI较低,且黄芪甲苷组<曲美他嗪组(P<0.05);各组大鼠心肌组织P-Cx3表达水平较低,且黄芪甲苷组<曲美他嗪组(P<0.05)。结论黄芪甲苷能够明显改善慢性心力衰竭大鼠心脏功能及血流动力学指标,抑制心肌细胞凋亡,该作用可能与下调心肌组织P-Cx3表达有关。
Objective To analysis the effect of Astragaloside IV on myocardial apoptosis and expression of Phosphorylated connexin 43 （P-Cx43） in rats with chronic heart failure （CHF）. Methods Sixty-three SD male rats were selected, and CHF rats model were established by abdominal aortic banding, the model rats were randomly divided into model group, Trimetazidine hydrochloride group, Astragaloside IV group, 21 in each group. Astragaloside IV group was given 60 mg/kg astragaloside IV once a day for intragastric administration; in the trimetazidine group, 10 mg/kg trimetazidine hydrochloride was given once a day for intragastric administration; The rats in the group and the control group were given 20 mg/kg distilled water once a day, and the rats in each group were continuously administered for 4 weeks. The cardiac function, hemodynamics indexes, heart weight / body mass ratio （HW/BW）, left ventricular mass / body mass ratio （LVW/BW）, myocardial cell apoptosis index （AI）, the expression of P-Cx3 in myocardial tissue were detected. Results After treatment, compared with model control group, the levels of LVEF in other groups were higher, and Astragaloside IV group〉Trimetazidine hydrochloride group （ P 〈0.05）. The IVSd, LVEDd, LVEDs levels were lower in other groups, and Astragaloside iv group〈Trimetazidine hydrochloride group, the difference was statistically significant （ P 〈0.05）. The LVSP, ＋dp/dtmax, -dp/dtmax levels were higher in other groups, and Astragaloside IV group〉Trimetazidine hydrochloride group （ P 〈0.05）. The LVEDP levels were lower in other groups, and Astragaloside IV group〈Trimetazidine hydrochloride group （ P 〈0.05）. HW/BW and LVW/BW levels of rats in other groups were lower, and Astragaloside IV group〈Trimetazidine hydrochloride group （ P 〈0.05）; the myocardial cells AI of rats in other groups were lower, and Astragaloside IV group〈Trimetazidine hydrochloride group （ P 〈0.05）. The expression of P-Cx3 in myocardium of rats were lower, and Astragaloside IV group〈Trimetazidine hydrochloride group （ P 〈0.05）. Conclusion Astragaloside IV can effectively improve cardiac function and hemodynamics parameters in rats with chronic heart failure, inhibit cardiomyocyte apoptosis, the effect may be related to the down-regulation of P-Cx3 expression in myocardium.
作者
赵岩
杨丹
于珊珊
ZHAO Yan;YANG Dan;YU Shan-shan.(Department of Preparation Center, The Second Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, Shenyang Liaoning 110034, China.)
出处
《临床和实验医学杂志》
2018年第20期2143-2147,共5页
Journal of Clinical and Experimental Medicine
基金
辽宁省科学技术计划项目(编号:2015225015)
