摘要
为研究鸡DIO2基因与鸡产热与低温调节间的关系,提取坝上长尾鸡肌肉总RNA,并反转录为c DNA,从Ensembl数据库下载DIO2基因序列(Gallus gallus-5.0 Chr 5:40,594,965-40,606,615),设计引物并进行RT-PCR扩增,PCR产物测序后进行生物信息学分析。确定了DIO2基因在鸡肌肉中表达,该基因编码区全长为840 bp,与NCBI数据库中的鸡DIO2基因同源性为99%,共编码279个氨基酸,亮氨酸含量最高为12.5%。聚类进化树结果表明,不同物种DIO2基因的脱氧核苷酸序列和氨基酸序列都表现高保守性。以上结果表明,DIO2基因在坝上长尾鸡肌肉中表达,且该基因在不同物种间高度保守。
To study the relationship between the DIO2 geneof chicken and the heat production and low temperature regulation of chicken,total RNA was extracted from the Bashang Long-tail chicken muscle andreverse transcribed into c DNA.The DIO2 gene sequence was downloaded from the Ensembl database.The primers were designed and PT-PCR amplification was performed. The PCR products of DIO2 gene were sequenced and analyzed with bioinformatics. The results showed that the DIO2 gene was expressed in chicken muscle and the gene coding region of DIO2 was 840 bp in length,and there＇s 99% homology with the DIO2 gene in the NCBI database.A total of 279 amino acids were encoded and the leucine content wasthe highest that was 12.5%. The results of phylogenetic tree showed highconservation in different species for deoxynucleotide sequence and amino acid sequence of DIO2 gene. The above results showed that the DIO2 gene was expressed in muscle of Bashang Long-tail chicken and had high conservation in different species.
作者
李笑
陈烨
付强
张配颖
牛文晓
黄天成
Li Xiao;Chen Ye;Fu Qiang;Zhang Pei-ying;Niu Wen-xiao;Huang Tian-cheng(College of Animal Science and Technology,HebeiAgricultural University,Hebei Baoding 071000)
出处
《生物化工》
2018年第4期84-87,共4页
Biological Chemical Engineering
