摘要
目的建立测定血浆泊沙康唑水平的高效液相色谱-串联质谱(HPLC-MS/MS)法,并应用于临床治疗药物监测。方法 HPLC色谱柱为Ultimate XB-C18。柱温50℃,流速0.8mL/min,流动相为含0.1%甲酸,以及2mmol/L乙酸铵的水溶液和含0.1%甲酸的甲醇溶液,梯度洗脱。质谱检测方式为ESI正离子模式,MRM扫描,监测泊沙康唑m/z 701.5~683.4作为定量离子,内标泊沙康唑-d4m/z 705.4~687.5作为定量离子。结果泊沙康唑水平在0.1~10.0μg/L范围内与峰面积线性关系良好(r=0.993 0),日内及日间精密度<5%,平均回收率为90%~103%。结论该方法简便、准确、快速,适用于泊沙康唑的血药浓度测定。
Objective To establish a HPLC-MS/MS method for determing of plasma posaconazole level in transplant patient,and to apply it in the drug monitoring of clinical treatment. Methods The HPLC chromatographic column was Ultimate XB-C18 column,the column temperature was 50 ℃,the flow rate was 0.8 mL/min,the mobile phase was the aqueous solution containing 2 mmol/L lammonium acetate and 0.1% formic acid,and methanol solution containing 0.1% formic acid.The gradient elution was performed.The mass spectrum detection method was ESI positive ion mode,MRM scanning,monitoring posaconazole m/z 701.5- 683.4 served as quantitative ion.The internal standard substance posaconazole-d4 m/z 705.4-687.5 served as quantitative ion. Results The posaconazole level had good linear relation with the peak area during the range of 0.1- 10 μg/L （ r =0.993 0）.The intra- and inter-day precision 〈5%,the average recovery rate was 90%-103%. Conclusion This method is convenient,accurate and rapid,and is suitable for the determination of posaconazole blood concentration.
作者
王磊
孙文利
刘红星
WANG Lei;SUN Wenli;LIU Hongxing(Hebei Yanda Lu Daopei Hospital,Langfang,Hebei 065201,China)
出处
《检验医学与临床》
CAS
2018年第19期2902-2905,共4页
Laboratory Medicine and Clinic
