摘要
目的:观察甘草附子汤对类风湿关节炎大鼠血清和滑膜组织中ASIC3和HIF-1α表达影响,探讨甘草附子汤治疗RA的相关机制。方法:40只SPF级Wistar大鼠随机分为4组,每组10只,除正常组外,其余组等量的不完全氟氏佐剂和牛Ⅱ型胶原乳化剂造模。甘草附子汤(9.75g/kg),醋酸地塞米松(7.5 mg/kg),连续灌胃20 d。分别拍摄大鼠足跖X射线,用ELISA检测大鼠血清中HIF-1α和ASIC3表达,免疫组化法检测关节滑膜组织中HIF-1α和ASIC3蛋白表达。结果:模型组大鼠血清和滑膜组织中ASIC-3和HIF-1α含量明显高于正常组(P<0.01),甘草附子汤组大鼠滑膜组织和血清中ASIC-3、HIF-1α含量明显低于模型组(P<0.01)。结论:甘草附子汤可以降低RA模型大鼠血清和滑膜组织中ASIC3、HIF-1α含量,可能是该方治疗RA的作用机制之一。
Objective： To observe the effect of Licorice and Aconite decoction（甘草附子汤） on the expression of ASIC3 and HIF-1α in serum and synovial tissue of rats with rheumatoid arthritis（RA）, and to explore and elaborate the related mechanism of Licorice and Aconite decoction in the treatment of RA. Methods： The40 SPF Wistar rats were randomly divided into 4 groups with 10 rats in each group. Except normal group, the other groups were modeled with incomplete Freund＇s adjuvant and bovine typeⅡcollagen emulsifier. Licorice and Aconite decoction 9.75 g/kg, dexamethasone acetate 7.5 mg/kg, continuous gavage for 20 days. X-rays of the rat＇s foot were taken respectively. The expression of HIF-1α and ASIC3 in the serum of the rats was detected by Elisa. The expression of HIF-1α and ASIC3 in the synovial tissue was measured by immunohistochemistry.Results： Compared with the normal group, the expression of ASIC-3 and HIF-1α in the serum and synovial tissue of the model group were significantly higher（P〈0.01）. Compared with the model group, the expression of ASIC-3 and HIF-1α in the synovial tissue and serum of the Licorice and Aconite decoction was significantly lower（P〈0.01）. Conclusion： Licorice and Aconite decoction can reduce the content of ASIC3 and HIF-1α in rat serum and synovial tissue of RA model rats, which may be one of the mechanisms of the treatment of RA.
作者
黄顺
林清华
王祖庆
李德仙
金泽旭
周兵望
王茂林
王永萍
HUANG Shun;LIN Qing-hua;WANG Zu-qing;LI De-xian;JIN Ze-xu;ZHOU Bing-wang;WANG Mao-lin;WANG Yong-ping(The First Affiliated Hospital of Guiyang College of Traditional Chinese Medicine,Guizhou Guiyang 550002,China;The Second Affiliated Hospital of Guiyang College of Traditional Chinese Medicine,Guizhou Guiyang 550002,China;Graduate School,Guiyang College of Traditional Chinese Medicine,Guizhou Guiyang 550002,China;School of Basic Medical Sciences,Guiyang College of Traditional Chinese Medicine,Guizhou Guiyang 550002 China)
出处
《中医药导报》
2018年第18期11-14,24,共5页
Guiding Journal of Traditional Chinese Medicine and Pharmacy
基金
国家自然科学基金(81460671)
贵州省大学生创新创业训练计划项目[贵中医大创合字(2015)26号]
贵阳中医学院大学生创新创业训练计划项目[贵中医大创合字(2016)19号]
贵州省教育厅千层次人才项目(贵中医[ZQ2015003])
