摘要
以樱桃型紫色番茄"砚紫1号"种子为试材,通过无菌处理获得无菌苗,用无菌苗的幼嫩子叶为外植体,研究了诱导愈伤组织分化及生根情况,以期建立高效的离体培养再生体系。结果表明:紫色番茄无菌苗子叶在MS+IAA 0.1mg·L^(-1)+6-BA 0.5mg·L^(-1)培养基条件下的愈伤组织诱导状态最佳;将愈伤组织进行暗培养处理,可诱导愈伤产生不定芽,其中最佳的培养基条件是MS+NAA 0.01mg·L^(-1)+6-BA 2.0mg·L^(-1);诱导再生的不定芽经过2周光培养接种至MS+NAA 0.05mg·L^(-1)培养基中,诱导生根良好,可获得完整的再生植株。
Cherry purple tomato ＇Yanzi No.1＇seeds were taken as experimental materials,and the sterile seedlings were obtained after sterile treatment;and then the immature cotyledons of sterile seedlings were used as explants for callus induction,differentiation and rooting were studied,and a complete tissue culture regeneration system was established.The results showed that purple tomato cotyledon cultured in MS＋IAA 0.1 mg·L^-1＋6-BA 0.5 mg·L^-1 was the best under the condition of callus induction medium;callus dark culture could induce callus to produce adventitious buds,the culture medium MS＋NAA 0.01 mg·L^-1＋6-BA 2.0 mg·L^-1 was the best;inducing regeneration of adventitious buds were cultured after 2 weeks of light in MS＋NAA 0.05 mg·L^-1 medium,rooting was fine,a complete regeneration system could be obtained.
作者
张园
张婉荣
林春
毛自朝
刘正杰
ZHANG Yuan;ZHANG Wanrong;LIN Chun;MAO Zichao;LIU Zhengjie(College of Agriculture and Biotechnology,Yunnan Agricultural University,Kunming,Yunnan 650201;Institute of Improvement and Utilization of Characteristic Resource Plants,Yunnan Agricultural University,Kunming,Yunnan 650201)
出处
《北方园艺》
CAS
北大核心
2018年第18期47-51,共5页
Northern Horticulture
基金
云南省应用基础研究计划资助项目(2015FB143)
关键词
紫色番茄
愈伤组织
不定芽
生根
再生体系
purple tomato
callus
adventitious bud
rooting
regeneration system
