摘要
为提高盐碱地中磷素利用率,分别从黄河三角洲及新疆盐碱地筛选得到解磷真菌各10株和6株,经18S r DNA鉴定,确定为曲霉菌属6株,青霉菌属9株,镰刀菌1株。解磷真菌的溶磷指数(SPI)为1.1~2.4;液体解磷量为11.4 mg L^(-1)~231.7 mg L^(-1);培养基的pH下降至3.40~4.97。线性关系分析表明,液体培养基的可溶性磷含量与pH呈显著负相关(r=-0.87)。选取解磷效果较好的菌株进行解磷动力学及耐盐能力测定。结果表明,解磷能力受时间的影响比较大,随着培养基内可溶性磷含量的增加,培养基的pH逐渐下降;所有菌株在NaCl浓度1.5 M条件能够生长,解磷真菌PSDX-8的最适生长NaCl浓度为1.2 M,其耐盐性最强。说明所筛选的解磷真菌具有在盐碱土壤中应用的潜力,这为开发高效磷肥提供了微生物资源。
To raise the utilization efficiency of phosphorus（P） in saline soil, 10 strains of phosphate-solubilizing strain of fungi（PSF）, named PFD-10, and 6 strains of PSF（PFD-6） were, respectively, isolated from the peanut rhizosphere in two typical saline areasof yellow river delta and Xinjiang, China. Among them, six strains belonged to Aspergillus spp, nine strains belonged to Penicillium spp., and one strain belonged to Talaromyces flavus according to 18 S r DNA sequence analysis. The capacity of dissolving tricalcium phosphate was analyzed by both agar plate and liquid culture.The soluble P indexvalue（SPI）was between 1.1 to 2.4 and all PSF had high potential for dissolving calcium phosphate ranging from 11.4 mg L^-1 to 231.7 mg L^-1. The pH value of culture medium had a significant negative correlation with soluble P（r =-0.87）. The PSF with better soluble P ability was selected to test its salt tolerance ability and soluble P dynamics. The culture timehad large effect on P dissolution ability. The pH value of culture mediumgradually decreased with the increase in the content of soluble P. All strains grew under the condition of 1.5 M NaCl, and the optimum growth of PSFD-8, which had the strongest salt resistance, was found under the condition of 1.2 MNaCl.Results showed that the selected PSF has a great application potential in saline soil, which would provide microbial resources forexploiting efficient P fertilizers.
作者
姜焕焕
祁佩时
王通
陈明娜
迟晓元
王冕
陈娜
潘丽娟
JIANG Huan-huan;QI Pei-shi;WANG Tong;CEHNG Ming-na;CHI Xiao-yuan;WANG Mian;CHEN Na;PAN Li-juan(Harbin Institute of Technology,Harbin 150000,China;Shandong Peanut Research Institute,Qingdao 266100,China)
出处
《土壤通报》
CAS
北大核心
2018年第4期856-861,共6页
Chinese Journal of Soil Science
基金
城市水资源与水环境国家重点实验室(2017DX15)
2014年国家"万人计划"青年拔尖人才
国家花生产业技术体系项目(CARS-14)
国家自然科学基金项目(31200211)资助
关键词
盐碱地
花生
解磷真菌
解磷特性
耐盐能力
Saline soil
Peanut
Phosphate-solubilizing fungi
Soluble phosphorus ability
Salt resistance
