摘要
目的探讨外泌体(exosome)来源linc-UFC1对胃癌细胞增殖和迁移的作用及机制。方法采用linc-UFC1高表达的胃癌细胞系BGC-823来源的外泌体处理胃癌细胞系MKN-45 48 h后,q RT-PCR检测linc-UFC1含量变化,细胞生长曲线和平板克隆形成实验检测细胞增殖能力,Transwell迁移实验检测细胞迁移能力;采用sh RNA敲减BGC-823细胞来源的外泌体中的linc-UFC1,作用于MKN-45细胞系,检测MKN-45细胞的linc-UFC1含量、增殖和迁移能力。结果与胃黏膜上皮细胞GES-1来源的外泌体相比,BGC-823细胞来源的外泌体中linc-UFC1的水平明显升高(q=56.12,P<0.01),而MKN-45来源的外泌体中linc-UFC1表达水平明显降低(q=5.98,P<0.01);经BGC-823外泌体处理后,与PBS组相比,MKN-45细胞内linc-UFC1水平明显升高(q=20.03,P<0.01),细胞增殖速率加快(q=25.84,P<0.01),平板克隆形成能力(q=48.73,P<0.01)和细胞迁移能力也明显增强(q=57.95,P<0.01);Linc-UFC1经敲减后,BGC-823外泌体中linc-UFC1水平明显降低(t=11.67,P<0.01);与对照外泌体处理组相比,外泌体上调胃癌细胞内linc-UFC1水平的作用被减弱(q=14.08,P<0.01),促进细胞增殖(q=14.45,P<0.01),平板克隆形成(q=18.58,P<0.01)及迁移(q=16.61,P<0.01)的作用被抑制。结论外泌体可通过转运linc-UFC1促进胃癌细胞增殖及迁移。
Objective To explore the roles and mechanisms of linc-UFC1 sourced from exosomes in the proliferation and migration of gastric cancer cells. Methods The exosomes derived from gastric cancer cell line BGC-823 which expressed high level of linc-UFC1 were incubated with gastric cancer cell MKN-45 for 48 hours. The level of line-UFC1 in MKN-45 cells was detected by qRT-PCR. Growth curve and plate colony formation assay were used to detect the proliferation ability of MKN-45 cells and transwell migration as- say was used to analyze the migration ability of the gastric cancer cells. The linc-UFC1 expression in exosomes derived from BGC-823 cells was knocked out by shRNA. MKN-45 cells were then treated with the exosomes, and the level of linc-UFC1, ability of cell prolif- eration and migration of MKN-45 cells were detected. Results Compared with the linc-UFC1 expression in exosomes derived from GES-1 epithelial cells of gastric mucosa, the linc-UFC1 expression in exosomes derived from BGC-823 calls was significantly up-regula- ted （q=56.12, P〈0.01） but it was down-regulated in those derived from MKN-45 cells （q=5.98, P〈0.01）. After treatment with exo- somes derived from BGC-823 cells, the level of linc-UFC 1 in MKN-45 cells was significantly increased compared with that of PBS treat- ment （ q = 20.03, P〈0.01 ） and the MKN-45 cells treated with BGC-823 exosomes exhibited strong proliferation （ q = 25.84, P〈0.01 ） , enhanced plate eoloning formation（q = 48.73, P〈O.O1 ） and migration ability （ q = 57.95 ,P〈0.01 ）. Following knockout of line-UFC 1 the expression of linc-UFC1 was significantly down-regulated in the exosomes derived from BGC-823 cells （t = 11.67, P〈0.O1 ）. The exosomes exhibited lower capacity to up-regulate line-UFC1 level in gastric cancer cell （q = 14.08, P〈0.01 ） , promote proliferation （ q = 14.45, P〈0.01 ）, plate colony formation （q = 18.58 ,P〈0.01 ） and migration （ q = 16.61, P〈0.01 ） of MKN-45 cells compared with the exosomes of control group. Conclusion Exosomes may promote the proliferation and migration of gastric cancer cells through the transfer of linc-UFC1.
作者
梁炜
臧雪燕
张宇
张鹏
钱晖
许文荣
张徐
LIANG Wei;ZANG Xneyan;ZHANG Yu;ZHANG Peng;QIAN Hui;XU Wenrong;ZHANG Xu(School of Medicine,Jiangsu University,Zhenjiang 212013,Jiangsu,China)
出处
《临床检验杂志》
CAS
CSCD
2018年第8期561-565,共5页
Chinese Journal of Clinical Laboratory Science
基金
国家自然科学基金(81672416
81572075)
江苏省重点研发计划(BE2015667)
江苏高校优秀科技创新团队
江苏省"333工程"项目
