摘要
目的:通过分析比较不同年龄人群的端粒相对长度,探索端粒长度改变与年龄的关系,为老年医学问题的深入研究提供数据和参考。方法:采用随机抽样的方法,从数家医院健康体检人群中抽取20岁以上者作为研究对象,收集其外周血白细胞作为检测标本。采用实时荧光定量PCR法进行端粒相对长度检测,分析年龄与端粒长度改变的关系。结果:共取样本1 022个,不同年龄组的相对端粒长度的差异有统计学意义(F=21.492,P<0.001),端粒长度与年龄呈负相关(r=–0.325,P<0.001),两者之间的回归方程为:y=–0.008x+1.772(P<0.001)。结论:健康人外周血白细胞端粒长度在各年龄段人群间有差异,且端粒长度随年龄增加逐渐缩短。
Objective： To explore the relationship between telomere length changes and age, and to provide data and reference for further study of geriatric medical problems.
Methods： Th e healthy people over 20 years old were chosen as subject from several hospitals by random sampling method, and their peripheral blood samples were collected. Th e relative length of telomere was detected by quantitative real-time PCR, and the relationship between age and telomere length was analyzed by statistical soft ware.
Results： A total of 1 022 samples were obtained. Th ere were signifi cant diff erences in the relative telomere length among diff erent age groups （F=21.492, P〈0.001）. Telomere length and age showed negative correlation （r=–0.325, P〈0.001）, the regression equation was y=–0.008x＋1.772 （x for age, y for the average telomere length, P〈0.001）.
Conclusion： Th e telomere length for peripheral blood leukocytes in healthy people varies between different age groups, suggesting that telomere length gradually decreases with age.
作者
叶兴
贺子强
邓鹏飞
魏毓
周建明
黄河浪
YE Xing, HE Ziqiang, DENG Pengfei, WEI Yu, ZHOU Jianming, HUANG Helang(Jiangxi Province Key Laboratory of Preventive Medicine, Nanchang University, Nanchang 330006, Chin)
出处
《中南大学学报（医学版）》
CAS
CSCD
北大核心
2018年第9期945-949,共5页
Journal of Central South University ：Medical Science
基金
国家自然科学基金(81360446)~~
