摘要
目的初步研究在骨疏康作用下,大鼠正畸牙移动性根吸收过程中MMP-2的表达规律。探讨骨疏康在根吸收发生发展过程中的作用,为正畸临床中牙根吸收的预防和治疗提供理论参考依据。方法选取60只雄性SD大鼠,随机分为实验组(30只)和对照组(30只),实验组进行骨疏康制剂灌胃,对照组采用蒸馏水灌胃,给药剂量为2.1 g/kg体重,于每天早晨灌胃一次。实验组和对照组再分别分为加力0、3、7、14、21、28 d组,每组5只,两组动物都给予0.6 N的力拉上颌右侧第一磨牙近中移动,建立大鼠正畸牙移动性根吸收模型,分别在实验的规定时间处死大鼠,HE染色观察牙根吸收形态学变化,免疫组化观察MMP-2在牙根吸收区的表达情况。结果两组大鼠不同牙移动时间牙根吸收指数比较发现,随着加力时间的延长,各组牙根吸收指数逐渐增大,根吸收从第三天开始出现,逐渐加重,14 d后根吸收速度减慢,组间相同时间点比较,骨疏康组根吸收程度轻于对照组,除0、3 d组牙根吸收指数无统计学意义(P>0.05),其余各组均有统计学差异(P<0.05)。免疫组化结果显示:MMP-2在加力0 d组表达较弱,随着加力时间的延长,MMP-2的阳性细胞数目逐渐增多,对照组和骨疏康组均在7 d时呈强阳性表达,达到高峰,14 d开始逐渐下降,骨疏康组在加力21 d时MMP-2呈弱表达。对照组在加力28 d时MMP-2呈弱表达,趋于正常组织的表达水平。结论 MMP-2是牙根吸收的主要炎性介质之一,参与了根吸收的过程,骨疏康可有效地抑制正畸牙移动根吸收的发生。
Objective To preliminarily study the expression of MMP-2 in the process of the movement of the orthodontic root of rats under the function of Gushukang, to explore its role in the development of root absorption, and to provide theoretical reference basis for the prevention and treatment of orthodontic clinical root absorption. Methods 60 male SD rats were randomly divided into experimental group （30） and control group （30）.The experimental group used Gushukang to fill the stomach, while the control group used distilled water to fill the stomach, the given dose was 2.1 g/kg body weight, lavaged once every morning. Experimental group and control group were respectively divided into strength 0 d, 3 d, 7 d and 14 d and 21 d and 28 d groups, each group with five. Two groups of animals were given to 0.6 N force to pull the right inaxillary first molar mesial movement.Orthodontic tooth mobility of rats root absorption model was set up. The experimental rats were put to death according to the time schedule respectively. HE staining was used to observe morphological changes of root absorption, and imnmnohistoehemistry was used to observe the expression of MMP-2 in the root absorption area. Results By the comparison of two groups of rats with different tooth movement time and root absorption index, it was found that with the extension of time, the root absorption indexes increased gradually, and the root absorption began to appear on the third day, then gradually increased. After 14 d root absorption rate slowed down, through the same time comparison between groups, root absorption degree of Gushukang group was lighter than the control group.Except for the fact that root absorption indexes of 0 d, 3 d groups had no statistical significance （P〉0.05）, the rest groups were statistically significant （P〈0.05）. Immunohistochemical results sho-ed that the expression of MMP-2 in the strength 0 group was weaker, with the extension of time, the number of MMP-2 positive cells increased. The two groupshad a strong positive expression on 7 d in peak, after 14 d began to gradually decline. The expression of MMP-2 of Gushukang group on 21 d was weak. In the control group, MMP-2 expressed weakly on 28 d and tended to the level of nor- mal tissue. Conclusion MMP-2 is one of the main inflammatory mediums absorbed by the root and is involved in the process of root absorption. The Gushukang is effective in inhibiting the absorption of orthodontic movement roots.
作者
刘红
王毅
詹娟
张真
万哲
张勤
LIU Hong;WANG Yi;ZHAN Juan;ZHANG Zhen;WAN Zhe;ZHANG Qin(Department of Stomatology,Affiliated Traditional Chinese Medicine Hospital,Xinjiang Medical University,Urumqi 830000,China)
出处
《口腔医学》
CAS
2018年第9期780-784,共5页
Stomatology
基金
新疆医科大学科研创新基金(ZYY201535)
关键词
正畸牙移动
根吸收
MMP-2
骨疏康
orthodontic tooth movement
root absorption
MMP-2
Gushukang
