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以党参炔苷和紫丁香苷为指标成分的纹党与白条党的鉴别 被引量:11

Identification of Wendang and Baitiaodang Based on Codonopatin and Syringin as Index Components
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摘要 目的以党参炔苷和紫丁香苷为指标性成分鉴别纹党和白条党。方法采用高效液相色谱法测定同一产地不同月份纹党炔苷含量,以Agilent1200高效液相色谱仪配有二极管阵列检测器(diode array detection,DAD),AT. Lichom C18液相分析色谱柱(250mm×4. 6mmid. 5μm,兰州中科凯特);柱温30℃,检测波长267nm,流动相为0. 3%磷酸-乙腈。结果纹党和白条党均含有党参炔苷,白条党中党参炔苷含量为1. 582 mg/g,纹党中党参炔苷含量为1. 136 mg/g,以单一的党参炔苷含量去评价质量优劣欠科学性。结论纹党中含有紫丁香苷,白条党中不含紫丁香苷,以紫丁香苷为指标可以鉴定白条党和纹党。 Objective To identify Wendang and Baitiaodang by taking Codonopatin and Syringin as index components. Methods The contents of codonopatin in different months of the same place of Wendang were determined by HPLC. The HPLC analysis was performed on an Agilent 1200 high -performance liquid chromatograph equipped with a diode array detection (DAD) and an AT. Lichom C18 liquid chromatography column 250 mm × 4.6mmid. 5μm, Lanzhou Zhongkekate) ; column temperature 30 ℃, detection wavelength 267nm, the mobile phase of 0.3% phosphoric acid - acetonitrile. Results Both Wendang and Baitiaodang contained Codonopatin, the content of Codonopatin in Baitiaodang was 1. 582 mg / g, and the content of Codonopatin in Wendang was 1. 136 mg / g. The quality of Codonopatin is of poor science. Conclusion The Wendang contains syringin, Baitiaodang does not contain syringin, with syringin as an index to identify the Wendang and Baitiaodang.
作者 胡春阳 戴迪 穆希琼 李富云 杨扶德 靳贵林 HU Chun-yang1 , DAI Di2 , MU Xi-qiong2, LI Fu-yun2 , YANG Fu-de2. , JIN Gui-lin3(1. Affiliated Hospital of Gansu University of Chinese Medicine, Lanzhou 730020, China;2. Gansu University of Chinese Medicine, Lanzhou 730000, China ;3. Tibetan Traditional Medical College, Lasa 850000, China)
出处 《时珍国医国药》 CAS CSCD 北大核心 2018年第8期1882-1885,共4页 Lishizhen Medicine and Materia Medica Research
基金 国家自然科学基金(81360622) 甘肃省自然科学基金项目(1107RJZA210) 甘肃省省属普通本科高校基本科研业务费专项资金(甘财教[2014]63号-5) 甘肃省兰州市人才创新创业专项(2014-2-15)
关键词 纹党 白条党 党参炔苷 紫丁香苷 鉴别 Wendang Baitiaodang Codonopatin Syringin Identification
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