摘要
目的：观察补肾化痰法经APN通路对肥胖PCOS大鼠卵巢GC增殖的调节作用研究。方法：采用来曲唑加高脂建立肥胖PCOS大鼠模型,补肾化痰复方治疗后,取卵巢组织行HE染色,免疫组化法检测AdipoR1/AdipoR2表达,realtime-PCR检测GC中p38MAPK、smad1/3、CCND2的mRNA表达水平。结果：与模型对照组比较,中药高、中剂量组卵巢GC中AdipoR1/2的表达增高（P〈0.05）;中药高、中剂量组卵巢颗粒细胞p38MAPK、smad1/3、CCND2表达量均增高（P〈0.05）。结论：补肾化痰法可能通过APN信号通路促进GC增殖,进而改善肥胖PCOS卵泡发育障碍,达到治疗作用。
Objective： To observe of the influence of granulosa cells（GC） multiplication of the method of kidney nourishing and phlegm removing（KNPR） through the APN pathway in obese PCOS rats. Methods： The rats were treated with the method of KNPR after modeling by Letrozole and high-fat. The ovarian tissues were taken out for HE staining,and the expressions of AdipoR1 and AdipoR2 were detected by immunohistochemical staining method,and the mRNA expressions of p38 MAPK,smad1/3 and CCND2 were detected by realtime-PCR. Results： Compared with model group,the AdipoR1/2 of high dose group（HDG） and middle dose group（MDG） was increased（P〈0. 05）. The mRNA expressions of p38 MAPK,smad1 and smad3 of HDG and MDG were increased（P〈0. 05）. Conclusion： The method of KNPR may promote the multiplication of GC by the APN pathway and improve the follicle developmental disorder of the obese PCOS to achieve the therapeutic effect.
作者
邓蒂斯
徐晓娟
黄映红
李宛静
雒芙蓉
张纯
梁小青
DENG Disi;XU Xiaojuan;HUANG Yinghong;LI Wanjing;LUO Furong;ZHANG Chnn;LIANG Xiaoqing(Clinical Medicine College of Chengdu University of TCM,Chengdu 610075,Sichuan,China;National Medical College of Chengdu University of TCM Wenjiang,Chengdu 611137,Sichuan,China;Basic Medical College of Chengdu university of TCM,Chengdu 611137,Sichuan,China;Clinical Medical College of Chengdu University of TCM,Chengdu 610075,Sichuan,China)
出处
《辽宁中医杂志》
CAS
2018年第9期1968-1971,I0005,共5页
Liaoning Journal of Traditional Chinese Medicine
基金
国家自然科学基金面上项目(81470199)
四川省教育厅基础项目(142A0315)
关键词
补肾化痰法
肥胖型PCOS
卵巢颗粒细胞
APN信号通路
细胞增殖
method of kidney nourishing and phlegm removing
obese PCOS
ovarian granulosa cells
APN pathway
cell mul-tiplication
